含脂食品辐照标志物2-烷基环丁酮检测技术研究进展

Progress in studies on the detection technique of 2-alkylcyclobutanones in irradiated lipid-containing foods

对比分析欧盟标准EN1785:2003方法,综述了近年来2-烷基环丁酮(2-Alkylcyclobutanones,2-ACBs)在提取、净化与检测等方面的研究进展。采用乙腈溶剂直接萃取含脂辐照样品中的2-ACBs→商品化固相萃取小柱净化→GC-MS检测是目前替代EN1785的潜力方法。固相微萃取、柱前衍生化及间接酶联免疫等方法具有快速、节约溶剂、低检出限等优点,但需要对操作条件进一步优化。

Recent advances in extraction, purification and detection methods of 2-alkylcyclobutanones （2-ACBs） were reviewed through comparing analysis of the EU standard （EN1785： 2003）. Using of direct extraction with acetonitrile, purification with commercial solid phase extraction columns and detection with GC-MS for 2-ACBs is probably the potential method which can be alternative of EN1785. The methods of SPME （Solid-phase microextraction）, pre-column derivation and indirect ELISA （Indirect enzyme-linked immunosorbent assay） have many advantages including saving analyzing time and organic solvent, and lowering detection limit. However, the relevant ooeratin~ conditions need to be further ootimized.