细胞松弛素B对山羊颞下颌关节盘细胞及细胞骨架肌动蛋白的影响

Effects of Cytochalasin B on Function and Actin Cytoskeleton of Goat Temporomandibular Joint Disc Cells

目的:肌动蛋白作为细胞骨架的重要结构蛋白,其与细胞形态和功能有着密切关系。因此本实验通过肌动蛋白抑制剂-细胞松弛素B检测细胞骨架完整性与山羊颞下颌关节盘细胞功能变化的关系。方法:检测不同浓度的细胞松弛素B对关节盘细胞增殖及形态的影响并得出最适浓度;观察最适浓度作用后细胞凋亡、粘附及肌动蛋白形态和mRNA表达的变化。结果:2μmol/L浓度的细胞松弛素B对关节盘细胞增殖活性具有明显抑制作用,但随培养时间延长抑制率明显降低,无促进凋亡作用;细胞粘附率明显下降;与对照组相比,处理组细胞突起结构逐渐消失,形态变圆,肌动蛋白纤维丝明显的断裂,荧光强度降低;肌动蛋白mRNA表达降低。结论:细胞松弛素B可能是通过破坏肌动蛋白的结构抑制了颞下颌关节盘细胞形态及功能。

Objective：To investigate the relationship between cytoskeletal integrity and the functional changes of temporomandibular join disc cells by cytochalasin B（CB）.Methods：Taking cell proliferation and morphology as evaluating parameters,different concentrations of CB were added to TMJ disc cells to get an optimal working concentration.Cells exposing to the optimal concentration of CB,apoptosis,adhesion and actin were observed.Results：CB of 2μmol/L obviously inhibited the proliferative activity of TMJ disc cells.However,as the culturing went on,the inhibition rate significantly reduced.No effect was observed with regard to cell apoptosis.The TMJ disc cell adhesion rate reduced.Compare with the control group,cells gradually lost ecptomas in the treatment group,becoming smoother;actin filaments obviously fractured and fluorescence intensity decreased.Moreover,actin mRNA expression reduced.Conclusion：CB may inhibit TMJ disc cell morphology and function by destroying the structure of actin.