牙周基础治疗对伴高脂血症牙周炎大鼠血清IL-6及牙槽骨吸收影响的研究

Effects of Periodental Non-surgical Treatment on Serum Il-6 and Alveolar Bone Absorption in Rats with Periodontitis and Hyperlipidemia

目的:建立慢性牙周炎(chronic periodontitis,CP)合并高脂血症(hyperlipidemia,HL)的SD大鼠模型并对其进行牙周基础治疗,观察血清白介素-6(interleukin 6,IL-6)炎症因子及牙槽骨的影响。方法:SD大鼠随机分为4组,对照组(A)、HL组(B)、CP组(C)、HL+CP组(D);进行相应的建模处理,从建模开始15周后随机处死B组大鼠1只,取颈动脉分叉血管组织进行油红O染色,观察到泡沫细胞形成,则建模成功。再将C/D组随机分为2小组,C1/D1为自然进程组,C2/D2为牙周基础治疗组,进行2次牙周干预,分别于干预前1周、第1次干预后1周、第2次干预后1、3、5周采血,酶联免疫吸附法测定血清IL-6含量。实验结束后处死所有大鼠,取单侧上颌骨,剥离牙龈,进行亚甲基蓝染色,使用电子数显卡尺在徕卡显微镜(16X)下测量离体上颌骨实验牙釉质牙骨质界至牙槽嵴顶(cementoenamel junction and alveolar bone crest,CEJ-ABC)的距离(第一、二磨牙共12个位点)作为牙槽骨吸收值以检测牙槽骨吸收情况。使用SPSS21.0软件对所得数据进行统计学分析。结果:血清IL-6含量C、D组明显高于A组(P<0.05),其中,C1/D1组随时间推移一直呈现上升趋势,C2/D2组则在第2次干预后1周血清IL-6含量达到高峰,随观察时间延长则逐渐下降并低于基线水平(P<0.05);牙槽骨丧失量:C、D组>A组(P<0.001),而C2/D2组较C1/D1组牙槽骨丧失略有改善,但差异无统计学意义;牙槽骨吸收与血清IL-6水平呈Pearson正相关关系(P<0.01)。结论:高脂血症可加重牙周炎病变,牙周干预后短期内表现为机体炎症反应加重,远期则可能因炎症因子水平的降低而减轻全身病变进程。血清IL-6水平升高后,牙周局部表现为牙槽骨的吸收量增加。牙周基础治疗一定程度上可改善伴或不伴有高脂血症的牙周炎大鼠牙槽骨丧失的进程。

Objective：To investigate effects of periodental non-surgical treatment on serum IL-6and alveolar bone absorption in rats with periodontitis and hyperlipidemia.Methods：The SD rats were separated into 4groups：control group（A）,HL group（B）,CP group（C）,and HL＋CP group（D）.After model establishment,group C and group D were randomly divided into group C1/D1（natural process group）and group C2/D2（scaling and root planning）according to random number table and received the corresponding oral intervention treatment respectively.Serum IL-6levels were determined by enzyme linked immunosorbent assay（ELISA）respectively one week before the intervention,one week after the first intervention,one,three,five weeks after the second intervention.The unilateral maxilla was collected and stained by methylene blue,and the distance between CEJ and ABC（cementoenamel junction and alveolar bone crest）was measured.Results：Serum IL-6of group C and D was obviously higher than that of group A（P0.05）.C1/D1 group showed an increased trend with time.The serum IL-6of C2/D2 group was peaked one week after the second intervention,then gradually decreased and was lower than the baseline（P 0.05）.The degree of alveolar bone loss was as follow：C,D group A group（P 0.001）.Serum level of IL-6and alveolar bone resorption were positively correlated（P0.01）.Conclusion：Hyperlipidemia can aggravate the periodontitis degree,and periodontal basic treatment exacerbates the body＇s inflammatory response in the short term.To some extent,periodontal basic treatment can decrease the alveolar bone loss in periodontitis rats with or without hyperlipidemia.