富血小板纤维蛋白修复犬股骨髁骨缺损的微观结构研究

Microstructure Studies on Platelet-rich Fibrin Repairing Bone Defects in the Beagle Dog

目的:通过建立beagle犬股骨髁的骨缺损模型,观察富血小板纤维蛋白(platelet-rich fibrin,PRF)诱导骨再生过程中新生骨的微观结构,评价PRF修复骨缺损的特点和优势,为PRF在诱导骨再生及颌骨重建的临床应用提供新的实验依据。方法:在实验犬股骨髁制备骨缺损,按Choukroun方法制备PRF膜,将PRF膜、Bio-Oss骨替代材料及自体骨骨松质随机填入3处骨缺损。12周、24周处死动物并取材,采用微焦点计算机断层摄影系统(microfocus computerizedtomography system,Micro-CT)检测分析新生骨的几何信息和结构信息。结果:PRF新生诱导骨骨小梁结构呈板状,厚度较厚,连续性和成熟度良好,数量稍少,接近自体骨组的成骨情况,优于Bio-Oss组;观察期内均数随时间延长,有向天然骨组衍变的趋势;PRF诱导成骨表现为向心性的特点。结论:PRF可诱导新生骨形成,成骨效果接近生理状态,表现为向心性成骨方式,具有良好的应用前景。

Objective：To observe the microstructure of new bone formed from PRF induction and evaluate the features and advantages of PRF on bone repair by the femoral condyle defect model inbeagle dogs,providingnew experimental basis forosteanagenesis and mandibular reconstruction.Methods：The PRF membrane was prepared by means of Choukroun.PRF,Bio-Oss bone substitute materials and autologous cancellous bone were randomly filled into bone defects in the femoral condyle of dog.At 12 weeks and 24 weeks,the dogswere sacrificedand the bone was retrieved.Micro-CT was used to detect and analyze geometry and structure information of new bone.Results：New bone formedfrom PRF hadplate-shape and thicker trabecular structure with good continuity and maturity,which was highly close to that inthe autogenous bone group and better than that in the Bio-Oss group.During the observation period,the average values were closeto natural bone.In bone regeneration process,PRF osteoinduction showed concentric features.Conclusion：PRF could induce the formation of new bone,microstructures of which were similar to physiological conditions and showed central regeneration,thus showing aprospect for clinical application.