口腔鳞癌细胞分泌的胞外体对其生物学行为的影响

Effects of Exosomes Secreted by Oral Squamous Cell Carcinoma Cells on Their Biological Behaviors

目的:研究口腔鳞状细胞癌细胞细胞株(OSC-4)分泌的胞外体对细胞生物学行为的影响。方法:培养OSC-4,从其上清培养液中分离提纯胞外体(exosome,EXO),CD9标记,将提取的胞外体作用于OSC-4,MTT检测胞外体对OSC-4增殖能力的影响,细胞划痕实验检测胞外体对OSC-4迁移能力的影响,transwill小室侵袭实验检测胞外体对OSC-4侵袭能力的影响。结果:细胞上清液提取物免疫金标结果可见金标记的CD9,即分离提纯物为胞外体;MTT检测结果显示胞外体作用后的OSC-4的增殖能力增强(P<0.05),细胞划痕实验结果显示其迁移能力增强和transwill小室侵袭实验结果显示其侵袭能力也增强(P<0.05)。结论:OSC-4分泌的胞外体可以促进口腔鳞状细胞癌细胞的增殖、迁移、浸润能力。

Objective：To study the effectsof exosomes secreted byoral squamous cell carcinoma cells（OSC-4）on their biological behaviors.Methods：Exosomesderived from OSC-4cells were separated from the medium.The effects of exosomes on the OSC-4cell proliferation,migration and invasion were detected by MTT,wound healing,andtranswellassays,respectively.Results：Gold-labeling CD9 was detected in the isolated exosomes.MTT assay showed that the OSC-4cells proliferation ability was increased after treated with exosomes（P0.05）.Wound healing and transwell assays results showed that the migration and invasion ability of OSC-4cells were also increased after treated byexosomes（P0.05）.Conclusion：Exosomes secreted byoral squamous cell carcinomacells couldpromote their proliferation,migration,and invasion.