bFGF与米贝拉地尔对人牙周膜成纤维细胞增殖的调节

Effect of bFGF and Mibefradil on Proliferation of Human Periodontal Ligament Fibroblasts

目的:研究成纤维细胞生长因子(bFGF,以下用B代表)与米贝拉地尔(Mibefradil,以下用M代表)这两种药物对人牙周膜成纤维细胞(HPLFs)增殖的调节作用。方法:B分10.0、20.0、30.0μg/L 3种浓度(分别为B1、B2、B3),M分2.5、5.0、10.0μmol/L 3种浓度(分别为M1、M2、M3),再按加药时间分为1d组、2d组和3d组,用WST法检测两种药物单独使用和按先后不同顺序联合使用后HPLFs的增殖率。结果:两种药物单独使用可分别起到促进和阻滞细胞增殖的效果,但是B作用时间过长细胞表现为增殖受阻滞。其中,B3作用1d组细胞增殖率最高(P<0.05),M2作用1d组细胞增殖率最低(P<0.05)。M仅对加用B3的细胞有明显阻滞作用,与单独使用B3的细胞增殖率有显著性差异(P<0.05)。另外,只有B1不能改变细胞加用M后增殖受阻滞的现象。所有联合用药后的细胞增殖率均与正常细胞增殖率无统计学差异。结论:bFGF与Mibefradil单独使用有各自的最佳作用浓度和作用时间。除过度增殖状态外,HPLFs增殖状态很难受到阻滞,并且处于增殖受阻滞状态的细胞很容易恢复增殖状态,以维持HPLFs不断处于增殖平衡状态。

Objective：To study the effect of basic fibroblast growth factors（bFGF,referred as B）and Mibefradil（referred as M）on proliferation of human periodontal ligament fibroblast（HPLFs）.Methods：B with three concentrations of 10,20 and 30ng/mL were respectively defined as B1,B2 and B3;M with three concentrations of 2.5,5and 10μmol/L were respectively defined as M1,M2 and M3;and B and M with three concentrations were respectively sub-divided into a 1-day group,a 2-day group and a 3-day group.The WST method was employed to detect the proliferation rate treated with one drug alone or combined usage of the two drugs in different orders.Results：B increased the proliferation of HPLFs,while M was on the contrary.The B3 1-day group showed the highest cell proliferation rate（P0.05）,and the M2 1-day group showed the lowest cell proliferation.There was significant difference in proliferation rate in B3 group combined with or without M（P0.05）.In addition,only B1 could not change the proliferation rate inhibited by the addition of M.There was no statistical difference in proliferation rate between combined usage groups and normal cells groups（P0.05）.Conclusion：bFGF and Mibefradil have their respective optimum acting concentration and acting time;Except the excessive proliferation state,the proliferation state of HPLFs is hard to block,and the blocking cells are easy to restore their proliferation state to continuously maintain the HPLFs in a balanced proliferation state.