头穴丛刺结合跑台训练对坐骨神经损伤大鼠轴突再生及S-100蛋白表达影响

Effect of Cluster Needling of Scalp Acupuncture Combined with Treadmill Training on Axonal Regeneration and Expression of S-100 Protein in Rats with Sciatic Nerve Injury

目的探讨头穴丛刺结合跑台训练对坐骨神经损伤大鼠轴突再生及S-100蛋白表达的影响。方法清洁级健康雄性Sprague-Dawley大鼠90只,随机分为空白组、假手术组、模型组、跑台组和头穴丛刺结合跑台组,每组18只,每组分为7 d、14d、21 d三个亚组。钳夹法造模,假手术组暴露坐骨神经但不钳夹。造模3 d后进行干预,跑台组进行跑台训练,头穴丛刺结合跑台组行头穴丛刺治疗结合跑台训练,空白组、假手术组及模型组除定时抓取外不做任何干预。各组相应时间点测定坐骨神经功能指数(SFI);取材后坐骨神经HE染色观察轴突生长情况,免疫组化染色检测S-100蛋白表达情况。结果各时间点跑台组及头穴丛刺结合跑台组SFI高于模型组(P<0.05),但低于假手术组和空白组;头穴丛刺结合跑台组SFI高于跑台组(P<0.05)。各时间点跑台组和头穴丛刺结合跑台组轴突生长情况均优于模型组,头穴丛刺结合跑台组优于跑台组。各时间点空白组和假手术组S-100蛋白均呈少量表达,跑台组、头穴丛刺结合跑台组S-100蛋白表达均高于模型组(P<0.05),头穴丛刺结合跑台组S-100蛋白表达高于跑台组(P<0.05)。结论头穴丛刺结合跑台训练能够促进S-100蛋白的表达,促进损伤神经轴突再生,提高坐骨神经功能。

Objective To explore the effect of cluster needling of scalp acupuncture combined with treadmill training on axonal regeneration and expression of S-100 protein in rats with sciatic nerve injury.Methods Ninety male Sprague-Dawley rats were randomly divided into blank group,sham group,model group,treadmill group and cluster needling of scalp point combined with treadmill group（combination group）,each group was further divided into 7,14 and 21 days subgroups,with 6 rats in each subgroup.The sciatic nerve of rats was clamped in the model group,the treadmill group and the combination group.The sham group was subjected to the same surgical procedure with sciatic nerve exposure but without crush injury.Three days after modeling,the treadmill group was treated with treadmill training,and the combination group was treated with cluster needling of scalp acupuncture and treadmill training,while the other groups were grabbed regularly,with no other intervention.The sciatic functional index（SFI） was tested,the condition of axon growth was observed with HE staining,and the expression of S-100 protein was examined with immunohistochemistry at each time point.Results At each time point,SFI was higher,the axon grew better,and the S-100 protein was higher expressed in the treadmill group and the combination group than in the model group（P〈0.05）,especially in the combination group（P〈0.05）.Conclusion Cluster needling of scalp acupuncture combined with treadmill training can accelerate the regeneration of axons and increase the expression of S-100 protein and improve sciatic function after sciatic nerve injury.