HPLC法测定复方补骨脂酊中3种成分的含量

Simultaneous Determination of Three Constituents in Compound Buguzhi Tincture by HPLC

目的 建立HPLC法测定复方补骨脂酊中补骨脂素、异补骨脂素和欧前胡素的含量。方法 色谱柱为Thermo Syncronis C18柱（250mm×4.6mm,5μm）,流动相为甲醇-乙腈-0.7%磷酸梯度洗脱,流速1.0m L·min-1,检测波长为300nm,柱温35℃。结果 补骨脂素、异补骨脂素和欧前胡素分别在4.0157~200.7846μg（r=1.0000）、4.0280~201.4000μg（r=1.0000）、0.7936~39.6806μg（r=1.0000）范围内线性关系良好;平均回收率（n=6）分别为106.17%（RSD=0.8%）、101.56%（RSD=1.3%）、102.64%（RSD=1.1%）。结论 该法简单准确、重复性好,为复方补骨脂酊的质量控制提供了一种准确可靠的检测方法。

OBJECTIVE To determine the contents of psoralen, isopsoralen and imperatorin in Compound Bu- guzhi Tincture by HPLC. METHODS The method was carried out on Thermo Syncronis C18 column （250mm×4.6mm,5μm）with a mobile phase of methanol-acetonitrile-0.7 % phosphoric acid gradient elution. The flow rate was 1.0mL·min-1 and the detection wavelength was set at 300nm. The column temperature was 35℃. RESULTS Psoralen ,isopsoralen and imperatorin was separated with a good linearity in the sample ranges of 4. 0157 - 200. 7846μg for psoralen ,4. 0280 -201. 4000μg for isopsoralen and 0. 7936 - 39. 6806μg for imperatorin. The average recoveries for psoralen,isopsoralen and imperatorin were 106. 17% （ RSD =0. 77% ）, 101.56% （ RSD = 1.3% ） and 102. 64% （ RSD = 1.1% ） , respectively. CONCLUSION The result was accurate and reproducible. The method can be used for the quality control of Compound Buguzhi Tincture.