腺苷对人胃癌MGC-803细胞增殖与凋亡的影响

Effects of adenosine on proliferation and apoptosis in human gastric cancer cell line MGC-803

目的 观察腺苷（adenosine）对人胃癌MGC-803细胞的增殖、凋亡的影响。方法 CCK-8法检测不同浓度腺苷（0.01-10 mmol/L）对胃癌MGC-803细胞增殖的影响。流式细胞仪检测腺苷（0.25、0.5、1 mmol/L）对细胞凋亡的影响。Western blot检测腺苷处理后细胞内凋亡相关蛋白和内质网应激相关蛋白的表达量。结果 腺苷能抑制胃癌MGC-803细胞的活力,并且呈现浓度和时间依赖性（P〈0.05）。腺苷（0.25、0.5、1 mmol/L）处理24 h后,细胞的总凋亡率显著升高（P〈0.01）。腺苷可以引起凋亡相关蛋白Caspase-3活化片段的蛋白表达量上调（P〈0.01）。腺苷能诱导内质网应激（ERS）相关蛋白Caspase-4活化片段、p-JNK、CHOP、GRP78的表达量上调（P〈0.05）。结论 腺苷可以抑制胃癌MGC-803细胞的增殖,并且促进凋亡,而内质网应激可能参与了腺苷引起的凋亡。

Objective To explore the influence of adenosine on proliferation and apoptosis in human gastric cancer cell line MGC-803. Methods MGC-803 cells were treated with different concentrations of adenosine （0.01 - 10 mmol/L）, and CCK-8 was used to observe cell proliferation. Flow cytometry （FCM） was used to analyze cells apoptosis after treatment of adenosine. Apoptosis protein Caspase-3 and endoplasmic reticulum stress （ERS） related proteins （casepase-4, p-JNK, CHOP, GRP78） were detected by Western blotting. Results Adenosine significantly inhibited the MGC-803 cells viability in a concentration- and time-dependent manner （P 〈 0.05 ）. After treatment with adenosine （0.25, 0.5, 1 retool/L） for 24 h, total apoptosis rate of cells significantly increased （P 〈0.01 ）. Expression of cleaved Caspase-3 was significantly up-regulated after treatment with adenosine （P 〈 0.01 ）. ERS related proteins （ casepase-4, p-JNK, CHOP, GRP78） were significantly up-regulated in adenosine treated group （P 〈 0.05 ）. Conclusion Adenosine can significantly suppress proliferation and induce apoptosis in MGC-803 cells, while endoplasmic reticulum stress is involved in adenosine-induced apoptosis.