巴戟天萃取物对环磷酰胺诱导睾丸损伤模型大鼠的影响

Effect of morinda officinalis extract on model rat with cytoxan induced testis injury

摘要目的：观察巴戟天萃取物（M0）对环磷酰胺（CTX）诱导睾丸损伤模型大鼠的影响及可能机制。方法：雄性SD大鼠，随机分为空白对照组、CTX＋NS组、CTX＋MO不同剂量组。以环磷酰胺构建大鼠睾丸损伤动物模型；巴戟天水提物用乙酸乙酯萃取后取水层成分给予每组治疗组灌胃。观察各组大鼠睾丸微细结构，比较卵泡刺激素受体（FSHR）蛋白及mRNA表达的差异。结果：光镜下观察可见CTX＋NS组明显病理改变，各治疗组的睾丸生精小管的结构明显改善，FSHRmRNA和FSHR蛋白表达明显高于CTX＋NS组，差异具有统计学意义。结论：巴戟天萃取物对环磷酰胺诱导睾丸损伤模型大鼠具有修复作用，可能与巴戟天萃取物能促进睾丸FSHR高表达有关，并以30g·kg-1·d-1浓度效果最佳。

Objective： To observe the effect of morinda officinalis extract （MO） on the model rat with cytoxan（CTX） induced testis injury and the possible mechanism. Methods： SD male rats were randomly divided into blank control, CTX＋NS and CTX-4-MO group. Models of impaired testis in the SD rats were made by intraperitoneal injection of CTX and treated by intragastric administration of MO at the concentration of 10, 20,30 and 40 g kg-1 d-1, respectively. Microstructure of testis tissue was observed by light microscopy. Expression of FSHR protein and mRNA was analyzed by Western blotting and PCR. Results： Light microscopy revealed obvious pathological changes of the testis tissue in the CTX A-NS and significantly ameliorated structures of the seminiferous tubles in the treatment groups. The expression of FSHR protein and mRNA in the treatment groups were dramatically higher than CTXA-NS group and the difference was statistically significant. Conclusion： Morinda officinalis extract can repair cytoxan-induced damage to rat testis, which is probably promote a high testis FSHR expressiorL The best effect is achieved at the concentration pf 30 g.kg-1 .d-1.