乙酰肝素酶在甲状腺乳头状癌患者组织及血清中表达的临床意义

Expression of heparanase in serum and tissues of patients with papillary thyroid carcinoma

目的探讨PTC患者组织和血清中乙酰肝素酶（heparanase，Hpa）的表达与临床意义。方法应用免疫组织化学法（SP）和酶联免疫法（ELISA）检测38例PTC及37例良性甲状腺结节患者组织及术前血清中Hpa蛋白的表达情况，同时检测30例正常人血清中Hpa蛋白的表达作为对照。结果PTC组织中Hpa的表达高于良性甲状腺结节（P〈O．01）．PTC组织中有周围淋巴结转移组Hpa的表达高于无周围淋巴结转移组（P〈0．05）。良性甲状腺结节患者血清中Hpa含量与正常对照组比较，差异无统计学意义（P〉0．05），PTC患者血清Hpa含量高于良性甲状腺结节组及正常对照组（P〈0．01），有无淋巴结转移患者血清中Hpa含量，差异无统计学意义（P〉0．05）。结论组织及术前血清中Hpa检测对门C诊断有一定价值，且组织中Hpa检测可用于判定PTC淋巴是否转移，而术前血清中Hpa检测尚不能用于判定PTC是否发生淋巴转移。

Objective To investigate the expression of heparanase （Hpa） in serum and tissue of patients with papillary thyroid carcinoma （PTC） and its clinical significance. Methods Immunohistochemical method （SP） and enzyme linked immunosorbent assay （ELISA） were used to detect the expression of Hpa protein in serum and tissues of 38 cases of PTC, 37 cases of patients with benign thyroid nodules, and 30 normal controls. Results Expression of Hpa in PTC was higher than that of benign thyroid nodules （P〈0.01）. Expression of Hpa in lymph node metastasis group was higher than that in group without lymph node metastasis （P〈0.05）. Compared with the normal control group, the content of serum Hpa in patients with benign thyroid nodules had no significant differ- ence （/：＇〉0.05）. Hpa content in serum of patients with PTC was higher than that of benign thyroid nodules group and the normal control group （P〈0.0I）. There was no statistically significant difference of the level of serum Hpa between groups with and without lymph node metastasis. Conclusions Detecting tissue and preoperative serum Hpa concentration is valuable for diagnosis of PTC. Hpa detection in tissues can be used to determine whether there was lymph node metastasis in PTC patients, while Hpa detection in preoperative serum can not judge whether there is lymph node metastasis.