急性早幼粒细胞白血病患者循环内皮细胞增殖能力及临床意义的研究

Primary Study of Proliferative Potential in Circulating Endothelial Cells from Acute Promyelocytic Leukemia Patients and Its Clinical Signiifcance

目的：探讨急性早幼粒细胞白血病（acute promyelocytic leukemia, APL）患者的循环内皮细胞（circulating endothelial cells, CECs）与健康者CECs在生物学特性上的差异，并分析其与PML/RARα融合基因的关系。方法免疫磁珠结合三色流式细胞仪分选计数APL患者的CECs。免疫荧光染色鉴定CECs表面CD31、CD144、VEGFR-2及CD133的表达情况，体外培养绘制其生长曲线，并测定CECs的集落形成率。结果 APL患者的CECs的数量及增殖能力明显高于对照组，16例APL患者CECs中CD133阳性率及集落形成率均高于对照组（P﹤0.05），其中CD34＋APL患者CECs表达CD133高达（50.3＆#177;9.2）%，其集落形成率是对照组的8倍。APL患者CECs数量及CD133阳性率均与PML/RARα融合基因呈正相关。结论精确计数并深入了解CECs可能有助于评价预后及设计治疗策略。

Objective To determine the biology feature of circulating endothelial cells （CECs） in acute promyelocytic leukemia （APL） patients compared to healthy donors, and analyze the relationship to expression of PML/RARαfusion gene.Methods CECs were sorted from peripheral blood by MACS and then counted by three color flow cytometry. The cells were identiifed by immunofluorescence staining for the expression of CD31, CD34, CD144, VEGFR-2 and CD133. These CECs were cultured in vitro and calculated to draw growth curve and determinate colony forming rate.ResultsThese CECs showed more increased quantity and proliferative potential as compared to healthy donors. The positive rate of CD133 and frequency of CECs is signiifcantly higher in APL patients than in healthy donors （P﹤0.05）. There was higher CD133 positive rate and more than 8-fold elevated frequency of CECs in CD34＋APL patients as compared to healthy donors. Higher CECs quantity and CD133 positive rate have positive correlation with expressions of PML/RARα fusion gene.Conclusion It may be helpful for evaluating prognosis and designing treatment strategy if we could accurately count and provide insight into these CECs.