摘要
在单因素试验的基础上,利用正交试验优化提取女贞花中总黄酮的工艺条件,并研究对DPPH自由基清除作用。结果表明:以0.5 g待测品为原料,按照1∶55(g/m L)料液比加入50%乙醇为提取剂,85℃恒温回流提取60 min为最佳提取工艺,女贞总黄酮提取率为12.57%(RSD=0.74);女贞黄酮对DPPH自由基清除率IC50值为11.97μg/m L,最大清除率82.06%。
To optimize the extraction of total flavonoids in Ligustrum flowers and its scavenging activity on DPPH free radicals,the factors influencing the process parameters were determined by specotrophotometric method. The results showed the optimum conditions of the extraction were as followes：0.5 g of samples were used as the raw material,50 % ethanol solution was used as the extraction solvent with a raw material to extraction solvent ratio of 1 ∶ 55（g/m L）,and the reflux extraction was carried out in 85 ℃water bath for 60 min. The extraction rate was 12.57 %（RSD=0.74）. The IC50 value of DPPH free radical scavenging rate by flavonoids in Ligustrum flowers was 11.97 μg/m L,and the maximum the clearance rate of DPPH free radical was 82.06 %.
出处
《食品研究与开发》
CAS
北大核心
2016年第14期42-45,67,共5页
Food Research and Development
基金
青岛农业大学高层次人才科研基金(663-1113320)
青岛农业大学大学生科技创新基金(2014305)
关键词
女贞花
总黄酮
DPPH自由基
Ligustrum flowers
total flavonoids
DPPH free radical
