天鹅源丙型副伤寒沙门菌感染实验小鼠的组织病理学及抗原分布

Histopathology and Antigen Distribution in the Mice Infected with Salmonella Paratyphi C Separated from Swan

为了研究天鹅源丙型副伤寒沙门菌感染致实验小鼠的组织病理学变化和其菌体抗原的组织分布,奠定菌株致病机制的研究基础,采用天鹅源丙型副伤寒沙门菌株口腔灌服感染实验小鼠,在感染后不同时点采集小鼠11个组织脏器制备石蜡切片,进行HE染色和免疫酶组织化学染色,对感染小鼠的组织病变及菌体抗原的分布进行观察。感染小鼠的心、肝、脾、肺、肾、肠道、脑等组织在感染3~6h后开始出现炎性反应,直至感染后14d;感染鼠回肠组织有显著的坏死、脱落及炎性损伤,右心室呈现脱落的血栓栓子,肺泡壁增厚、血管充血、水肿、炎性细胞浸润和支气管肺炎,多脏器变性、坏死、炎性细胞浸润,多脏器组织可见＂伤寒小结＂。感染3h,除脑外,其他被检脏器皆检出细菌抗原,除感染途径肠道细菌抗原含量极高,脾、心、肺、肝的阳性信号也很强,且主要存在组织的细胞质中。研究结果提示天鹅源丙型副伤寒沙门菌能感染实验小鼠,侵嗜小鼠多个脏器,长时间不同程度地引起多脏器变性、坏死及炎性反应。

To learn histopathology of the mice infected with Salmonella ParatyphiC,clarify antigen distribution in tissue of the mice,and lay the groundwork of pathogenic mechanism,mice were infected with Salmonella Paratyphi C by drench.After being infected,11 tissues of the mice were sampled,embedded in paraffin,and dyed by HE and immune enzymatic technique.Three to six hours post challenge,inflammatory reaction were observed in heart,liver,spleen,lung,kidney,intestinal tract and brain,and lasted until 14 dpost challenge.Necrosis,fall-off and inflammatory injury occurred in ileum,thrombus were found in ventriculus dexter.Alveolar walls were thickened,and vascular congestion,edema and inflammatory cells infiltration were observed.Chronic fibrous pneumonia,alveobronchiolitis,acute glomerulonephritis,and denaturation,necrosis,inflammatory injury and typhoid nodule in several organs were observed.Three hours after being challenged,antigen was detected in the sampled organs except brain.Besides extremely high quantity of antigen in the intestinal tract（infection route）,the strongest positive signal were also detected in spleen,heart,lung and liver,which mainly existed in cytoplasm of the tissues.These results indicate that Salmonella Paratyphi C separated from swan can infect mice,encroach many organs of mice,and lead to denaturation,necrosis and inflammatory injury in the organs to different extent in long time.