摘要
建立了HPLC法同时测定补中益气丸中阿魏酸、橙皮苷和甘草酸的含量。采用AgilentZORBAX SBC18（250mm×4．6mm，5μm）色谱柱，以乙腈-0．05％磷酸水溶液为流动相进行梯度洗脱；流速：1．0mL·min^-1；检测波长：340nm；柱温：30℃。结果显示，阿魏酸的进样量在0．064—1．6μg范围内（r=0．9992）、橙皮苷的进样量在1．6—40μg范围内（r=0．9997）、甘草酸的进样量在1．6—40μg范围内（r=0．9992）线性关系良好，阿魏酸、橙皮苷和甘草酸的平均回收率分别为99．90％（RSD=2．07％，n=6）、101．25％（RSD=1．40％，n=6）和100．89％（RSD=2．20％，n=6）。该方法简便快捷，重现性好，结果准确，可用于补中益气丸的质量控制。
A method for the determination of ferulic acid, hesperidin and glycyrrhizic acid in Buzhongyiqi pills was established. The separation was performed on a Agilent ZORBAX SBC18 column （250 mm×4. 6 mm, 5 μm） with acetonitrile-0.05% phosphoric acid solution as mobile phase with gradient elution at the flow rate of 1.0 mL · min^-1. The detection wavelength was set at 340 nm, and the column temperature was 30 ℃. The linear range of erulic acid, hesperidin and glycyrrhizic acid was 0. 064 - 1.6 μg （ r = 0. 9992 ）, 1.6 - 40μg （ r = 0. 9997 ） and 1.6 - 40 μg （ r = 0. 9992）, the average recovery rates were 99.90% （ RSD = 2.07%, n = 6）, 101.25% （ RSD =. 40%, n = 6） and 100. 89% （RSD = 2. 20% , n = 6）, respectively. The established HPLC method is proved to be convenient, reproducible, precise and suitable for the content determination of Buzhongyiqi pills.
出处
《广州化工》
CAS
2016年第16期139-141,172,共4页
GuangZhou Chemical Industry
