刺梨中SOD的提取和酶活测定

Extraction and Activity Determination of SOD in Rosa roxburghii Tratt

刺梨原产于我国西南地区,以贵州省为主,是目前发现超氧化物歧化酶(SOD)含量最高的水果[1]。本实验采用邻苯三酚自氧化法,分别测定刺梨鲜果、糖渍刺梨、刺梨干冲泡的饮品和刺梨酒中的SOD酶活。实验结果表明,刺梨鲜果和糖渍刺梨的SOD酶活分别为2140 U/g和3716.2 U/g;刺梨干冲泡的饮品、刺梨酒的SOD酶活分别是308.2 U/m L、368.1 U/m L。经比较发现,刺梨果实用糖腌制后SOD活性提高1.7倍左右,而制成饮品后SOD的酶活变化不大。

Rosa roxburghii Tratt is a kind of fruit native to southwest of China, mainly in Guizhou province. Its super oxide dismutase （SOD） content is the highest among fruits. Pyrogallol autoxidation method was used to determine the SOD activity of fresh fruits and candied fruits, drinks and wine. The results showed that the SOD activity of fresh fruits and candied fruits were 2140 U/g and 3716. 2 U/g, respectively. The SOD activity of drinks and wine were 308.2 U/mL and 368.1 U/mL, respectively. It could be found that the SOD activity of the sugared fruits was 1.7 times that of the fresh fruits, while the SOD activity of the drinks and wine were similar.