二氢杨梅素对衰老模型小鼠的改善作用

Improvement effects of dihydromy ricetin on D-galactose-induced aging mice

目的:研究二氢杨梅素对衰老模型小鼠的改善作用。方法:将50只小鼠随机分为正常组、模型组和二氢杨梅素(250,500,1 000 mg·kg-1)给药组,皮下注射5%D-半乳糖,注射量为25 mL·kg-1,每天1次,连续42 d复制小鼠衰老模型。造模同时给药,各组小鼠分别给予相应剂量的二氢杨梅素,连续42 d。末次给药后2 h对小鼠学习记忆能力进行测定,然后测定小鼠脑组织丙二醛(MDA)、脂褐素(LF)含量与超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果:与正常组相比,模型组学习记忆能力出现显著下降(P<0.01),MDA、LF水平显著升高(P<0.01),SOD和GSH活性显著降低(P<0.01)。与模型组相比,给予二氢杨梅素后,小鼠学习记忆能力显著提高(P<0.01),MDA、LF水平显著降低(P<0.01),SOD和GSH活性显著升高(P<0.01)。结论:二氢杨梅素能够有效提高机体的抗衰老能力,其机制可能是与二氢杨梅素提高机体的抗氧化能力有关。

OBJECTIVE To study the improvement effects of dihydromy ricetin on D-galactose-induced aging mice.METHODS All the rats were randomly divided into normal control group,model group and dihydromy ricetin（1 000,500,250 mg·kg-1）groups,5% D-galactose（25 mL·kg-1）was sc given to mice once a day for consecutive 42 d to establish aging models,while dihydromy ricetin（1 000,500,250 mg·kg-1）groups were given dihydromy ricetin for 42 d,then the ability of learning and memory and the content of MDA and LF and the activity of SOD and GSH were detected.RESULTS Compared with normal control group,the content of MDA and LF were increased significantly and the activities of the SOD and GSH of the mice in model group were decreased,the ability of learning and memory were decreased.Compared with model group,the content of MDA and LF were decreased significantly and the activities of the SOD and GSH of the mice in dihydromy ricetin group were increased,the ability of learning and memory were increased.CONCLUSION Dihydromy ricetin has improvement effects on Dgalactose-induced aging mice and its mechanism may be related to the enhancement of the activity of antioxidant enzyme and the improvement in oxidation resistance.