内生真菌对油樟悬浮细胞培养的影响

Effects of fungal endophytes on cell suspension culture of Cinnamomum longepaniculatum

该文研究了内生真菌YG42、YG71、YY11和YY26发酵液,对油樟悬浮细胞的生长量及挥发性代谢产物积累量的影响。结果表明:4种内生真菌对油樟悬浮细胞的生长均有抑制作用,抑制强度随发酵液添加量的增加而加强。4种内生真菌对油樟悬浮细胞挥发性代谢产物积累总量及1,8-桉叶油素、γ-叶松油烯和α-油松油醇3种油樟油组分物质积累量的影响多表现为低浓度促进高浓度抑制的趋势。其中,1%添加量的YG42和YY26及0.25%添加量的YY11对悬浮细胞总挥发性代谢产物积累的促进作用相当且最强,其积累量分别是空白组的2.00、1.95、2.01倍;0.25%添加量的YG71对1,8-桉叶油素积累的促进作用最强,其积累量为空白组的11.03倍;0.25%添加量的YG71和YY26对α-松油醇积累的促进作用相当且最强,其积累量分别为空白组的1.72和1.81倍;对于γ-松油烯的积累,在空白组中未检测到其峰值,4种真菌诱导子对γ-松油烯的产生有诱导作用,诱导的最大峰面积为0.19,诱导菌是0.25%添加量的YG71。该研究结果为充实内生菌影响香料植物挥发性代谢产物合成理论奠定了基础,也为生产上内生真菌提高油樟油中有用物质组分含量措施的采用提供了依据。

We studied the effects of fungal endophytes YG42,YG72,YY11 and YY26 on cell growth and volatile of secondary metabolites accumulation in suspension cultures of Cinnamomum longepaniculatum. The results showed that four kinds of fungal endophytes had obvious inhibitory effects on C. longepaniculatum cell growth,and the denser the fermentation fluid was,the stronger inhibitory effects they had. The trend of the effects that the four kinds of endophytic fungi had on the total volatile of secondary metabolites accumulation and C. longepaniculatum oil component 1,8-cineole γ-terpinene and α-terpineol accumulation in suspension cultures of C. longepaniculatum was promoted at a low concentration and inhibition at a high concentration. The promotion effects of 1% YG42,YY26 and 0.25% YY11 on the total volatile of secondary metabolites accumulation were equivalent,and they had the strongest promoting effects on the total volatile of secondary metabolites accumulation,which were 2.00,1.95 and 2.01 times of the control respectively. The0.25% YG71 had the strongest promoting effects on the 1,8-cineole accumulation,which was 11.03 times of the control. The 0.25% YG71 and YY26 had the strongest promoting effects on the a-terpineol accumulation,which were 1.72 and 1.81 times of the control respectively. γ-terpinene was not detected in the control. Four kinds of endophytic fungi could induce γ-terpinene production,the biggest peak areas were 0.19,and the endophytic fungi was 0.25% YG71. This research will enrich the theory that fungal endophytes affect the volatile of secondary metabolites in aromatic plant,and provide experimental evidence for the use of endophytic fungi to increase the amount of useful component in C. longepaniculatum oil accumulation in production.