红细胞悬浮液多参数光谱测量方法研究

Determination of multiple optical parameters of red blood cell suspension

目的提出了一种基于散射光空间分布信号、无需积分球装置测量计算红细胞(RBC)悬浮液多参数光谱的新方法。方法利用光调制技术和独立光电传感器相结合采集血液样品空间分布光强信号,获得由漫反射率、漫透射率和准直透射率组成的特征信号测量值,并用其作为输入参数,通过基于蒙特卡洛(Monte Carlo)仿真模型和微扰算法的逆计算,获得被测样品吸收系数、散射系数和各向异性因子的光谱数据。结果用该法测量了粒径2.6μm聚苯乙烯微球悬浮液的多参数光谱,并与Mie理论计算结果进行了校验。在此基础上,进一步测量了7.1%浓度RBC悬浮液的多参数光谱,获得的光谱数据与文献中利用积分球结合蒙特卡洛结果基本一致。结论基于空间特征光信号测量并计算RBC悬浮液多参数光谱方法,将为深入研究非侵入性定量检测RBC和游离血红蛋白含量提供一种快速、准确的新途径。

Objective To develop a measurement method for determination of optical parameters of a red blood cell（ RBC） suspension based on the measurement of spatial scattered light signals without using an integrating sphere. Methods Multiple independent photoelectric sensors and light intensity modulation were used to obtain the measured values of diffuse reflectance,diffuse transmittance and collimated transmittance. The measured data results were imported into a Monte Carlo simulation based RTE to inversely determine the absorption coefficient,scattering coefficient and anisotropy factor of the measured sample using a new perturbation method. Results The described measurement method was applied to determine the optical parameters of a polystyrene microsphere suspension with a mean diameter of 2. 6 μm,and the results were essentially consistent with the calculated optical parameters by Mie code. Then,the RBC suspension was used for testing optical parameters,and the results were basically consistent with the parameters in the literature. Conclusion The system based on the measurement of spatial scattered light signals without using an integrating sphere will provide a quick and accurate approach for quantitative analysis of free hemoglobins and RBC suspensions.