荣骨定痛膏对骨性关节炎大鼠IL-1β、NO及氧自由基代谢的影响

The Influence of RongGu DingTong Ointments on IL-1β, NO and Oxygen Free Radical Metabolism of OA Rats

目的:探讨荣骨定痛膏对骨性关节炎模型大鼠白介素1β(IL-1β)、一氧化氮(NO)及氧自由基代谢的影响。方法:采用注射4%木瓜蛋白酶复制骨性关节炎大鼠模型,通过观测膝关节功能,血清IL-1β、关节液NO与NOS、关节组织氧自由基代谢等指标,探讨荣骨定痛膏对骨性关节炎的治疗作用及作用机制。结果:荣骨定痛膏可明显减小骨性关节炎大鼠关节滑膜肿胀厚度,扩大膝关节的活动范围,降低血清IL-1β、关节液NO及NOS的水平,提高滑膜组织中SOD的活性而降低MDA的含量。结论:荣骨定痛膏对骨性关节炎模型大鼠具有一定治疗作用,抑制IL-1β诱导的NO的产生而发挥抗氧化、抑制滑膜细胞的凋亡是其治疗骨性关节炎的作用机制之一。

Objective： To discuss the effects ofRongGu DingTong ointment on IL-1β, NO and oxygen free rad- ical metabolism of the rats with osteoarthritis （OA）. Methods： OA rat model was established by injecting 4% papain, therapeutic effects and its mechanism ofRongGu DingTong ointment were studied by observing knee joint function, the contents of IL-1β, NO and NOS, and oxygen free radical metabolism in joint tissue. Results： RongGu DingTong ointment could reduce the thickness of joint synovial swelling of OA rats, increase the range of motion of knee joint, decrease the levels of IL-1β, NO and NOS, and improving SOD activity in synovial tissue therefore to decrease the contents of MDA. Conclusion： RongGu DingTong ointment is effective in treating OA rats to a certain degrees, one of its mechanisms is inhibiting NO generation induced by IL-1β, hence to develop antioxidant functions and inhibit the apoptosis of synovial cells.