气相色谱法测定辛芷滴鼻液中龙脑和异龙脑的含量

Determination of Borneol and Isoborneol in XinZhi Nasal Drops by Gas Chromatography

目的：建立辛芷滴鼻液中龙脑和异龙脑的含量测定方法。方法：采用气相色谱法测定,DB-WAX色谱柱（30 m×0.53 mm,1.0μm）,载气为氮气,分流比为10∶1,柱温为140℃,进样口温度为180℃,检测器温度为200℃。结果：龙脑和异龙脑分别在17.6-282.0、11.8-188.4μg/m L范围内呈良好的线性关系（r=0.999 9、r=0.999 9,n=6）;平均加样回收率分别为98.91%和99.45%（RSD为1.36%、1.53%,n=9）。结论：本法简便、准确、灵敏度高、重复性好,可用于辛芷滴鼻液的质量控制。

Objective： To establish content determination method of borneol and isoborneol in XinZhi nasal drops. Methods： Gas chromatography was adopted to detect DB-WAX chromatographic column （30 m×0.53 mm, 1.0 μm）, carrier gas was nitrogen, split ratio 10：1, column temperature 140℃, inlet temperature 180℃ and detector temperature 200℃. Results： Borneol and isoborneol showed better linear relationship in the ranges between 17.6 and 282.0, 11.8 and 188.4 μg/mL （r=0.999 9, r=0.999 9, n=6）; average recovery rates were 98.91% and 99.45% respec- tively （RSD=1.36%, 1.53%, n=9）. Conclusion： The method, simple, accurate, high sensitivity and good reproducible, could be used for quality control of XinZhi nasal drops.