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乙型肝炎病毒蛋白诱导白细胞介素32表达情况的研究 被引量：1

Expression of IL-32 induced by hepatitis B virus protein
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摘要 目的探讨乙型肝炎病毒蛋白诱导白细胞介素32(IL-32)的表达以及临床意义。方法用Lipofectamine2000 ^(TM)介导转染Hep G2细胞。转染48 h后,采用聚合酶链反应(RT-PCR),蛋白免疫印迹法(Western blot)分别检测Hep G2细胞中IL-32的m RNA及蛋白的表达量。结果 RT-PCR结果显示转染HBV的Hep G2细胞中IL-32 m RNA的表达量比转染空载体(未转染)的细胞中IL-32 m RNA高2.3倍;Western blot结果显示与空载体转染的Hep G2细胞比较,转染p IRES2-HBV-EGFP的Hep G2细胞中IL-32蛋白质表达增高,且差异有统计学意义(P<0.05)。结论 HBV促进IL-32蛋白的表达,从而证实乙型肝炎的严重程度与IL-32的表达有关。 Objective To investigate whether protein of hepatitis B virus induced the expression of IL-32. Methods HepG2 was mediated transfected by Lipofectamine 2000 TM. After 48 hours, the expressions of IL-32 mRNA and protein in HepG2 cells were detected by RT-PCR and western blot respectively. Results RT-PCR result showed expressions of IL-32 mRNA in HepG2 cells transfected with HBV were 2.3 times higher than the cells transfected with empty vector （non-transfected） . Western blot result showed that, compared with the IL-32 protein in untransfeeted HepG2 cells, expression of IL-32 protein in transfected pIRES2-HBV-EGFP HepG2 cells was increased, and the difference was statistically significant （P 〈 0.05）. Conclusion The expression of IL-32 mRNA, IL-32 protein in HepG2 cells is promoted by HBV.
作者 张晶晶 谢永富 胡建峰 刘会晶 孙宏勋
机构地区 河北医科大学第三医院检验科
出处 《中国现代医学杂志》 CAS 北大核心 2016年第16期35-38,共4页 China Journal of Modern Medicine
关键词 乙型肝炎病毒蛋白 IL-32 表达 研究 hepatitis B virus protein IL-32 expression study
分类号 R575.1 [医药卫生—消化系统]
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参考文献14

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二级参考文献36

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中国现代医学杂志
2016年 第16期

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