肠杆菌科细菌碳青霉烯酶基因的检测

Detection of carbapenemase gene in Enterobacteriaceae

目的了解余姚地区耐碳青霉烯类药物肠杆菌科细菌的耐药情况和碳青霉烯酶耐药基因类型。方法收集2014年3月至12月耐亚胺培南和厄他培南的肠杆菌科细菌18株,进行Hodge试验确认。对于阳性试验菌株采用PCR法检测bla_(KPC)、bla_(NDM-1)、bla_(MH)、bla_(GES)、bla_(SME)、bla_(NmcA)和bla_(SHV-38)七种基因。结果 18株耐碳青霉烯类肠杆菌科细菌经改良Hodge试验确认阳性11株,占61.1%。经PCR检测显示11株均携带有bla_(KPC)基因,其中肺炎克雷伯菌6株,大肠埃希菌3株,阴沟肠杆菌2株。结论余姚地区耐碳青霉烯类药物肠杆菌科细菌的耐药机制主要是bla_(KPC)型碳青霉烯酶。

Objective To investigate the drug resistance and Carbapenem resistance gene type of carbapenem-resistant Enterobacteriaceae bacteria. Methods Collected 18 strains of resistant to imipenem and ertapenem Enterobacteriaceae bacteria, Hodge test conducted to confirm. For positive test strains using PCR method to detect blaKPC、blaNDM-1、blaMH、blaGES、blaSME、blaNmcA和blaSHV-38 genes. Results 18 strains of carbapenem-resistant Enterobacteriaceae confirmed by the modified Hodge test positive for 11 strains, accounting for 61.1%. The PCR test showed that all 11 strains were carried blaKPC, among the 6 strains of Klebsiella pneumoniae, 3 strains of Escherichia coli, 2 strains of Enterobacter cloacae. Conclusions The region carbapenems-resistant Enterobacteriaceae bacterial resistance mechanisms is mainly blaKPC type carbapenemases.