miRNA-27a-3p在结直肠癌组织及外周血中表达的临床意义

Clinical value of miRNA-27a-3p expressed in peripheral blood and cancer tissues of colorectal cancers

目的 检测miRNA-27a-3p在结直肠癌组织、肝脏转移灶及外周血中的表达水平并分析靶基因,研究其表达变化与临床病理特征的关系,初步探讨其在结直肠癌发生发展过程中的临床意义.方法 选取78例结直肠癌患者为研究对象,采用实时定量RT-PCR检测外周血、结直肠癌组织、癌旁组织及肝脏转移组织中miRNA-27a-3p表达量,分析其在不同组织中的表达差异,通过靶基因网站预测法阐述miRNA-27a-3p在结直肠癌患者中的临床意义,预测miRNA-27a-3p作用的靶基因,并证实miRNA-27a-3p与靶基因相关性.结果 结直肠癌组织中miRNA-27a-3p表达水平为3.13±1.72明显高于配对癌旁组织的1.06±0.42及对照组的0.68±0.27（P＜0.05）.其中27例肝脏转移肿瘤组织的miRNA-27a-3p表达水平为3.48 ±1.15,明显高于配对结直肠癌组织2.34±1.03（P＜0.05）及癌旁组织1.14±0.81（P ＜0.05）.结直肠癌患者组织中及外周血中miRNA-27a-3p的表达水平在不同年龄、性别、血癌胚抗原（CEA）水平、肿瘤部位、组织学类型之间差异均无统计学意义（P＞0.05）,在淋巴结有无转移、肝脏有无转移、TNM分期之间的差异有统计学意义（P＜0.05）.miRNA-27a-3p与靶基因高尔基膜蛋白73（GP73）表达呈负相关性.结论 外周血及结直肠癌组织中miRNA-27a-3p的高表达患者TNM分期更晚,且具有更高的发生肝脏转移的风险,miRNA-27a-3p可能通过调控靶基因GP73参与结直肠癌的发生发展,外周血中miRNA-27a-3p的检测对于结直肠癌患者具有潜在的辅助诊断及预估肝脏转移的临床意义.

Objective To investigate clinical value of miRNA-27-3p expressed in colorectal cancers,liver metastasis,and the peripheral blood,and analyze its target genes.Methods Among 78 cases of colorectal cancer patients,sera,colorectal cancers,and liver metastases were used to detect miRNA-27a-3p expressions with real-time quantitative polymerase chain reaction （RT-PCR） and analyze its clinical significance.We predicted miRNA-27-3p target genes,and confirmed the target genes and their correlation between miRNA-27a-3p and target genes.Results The expression level of miRNA-27a-3p in 78 cases of colorectal cancers was 3.13 ± 1.72,which was significantly higher than that in matched adjacent cancer tissues （1.06 ± 0.42） and control group （0.68 ± 0.27） （P 〈 0.05）.The expression level of miRNA-27a-3p in 27 cases of liver metastases was 3.48 ± 1.15,which was significantly higher than that in paired colorectal cancer tissues （2.34 ± 1.03） （P 〈0.05） and adjacent tissues （0.81 ± 1.14） （P 〈0.05）.Expression levels of miRNA-27a-3p in patients with colorectal cancer tissues and peripheral blood had no significant difference in age,sex,serum carcinoembryonic antigen （CEA） levels,tumor location,and pathological types （P 〉 0.05）;however,it had positive significant difference in lymph node metastasis,liver metastasis,tumor node metastasis （TNM） staging （P 〈 0.05）.The expression of miRNA-27a-3p was negatively correlated with the expression of target gene GP73.Conclusions The higher expression was miRNA-27a-3p in peripheral blood and colorectal cancer tissues,higher risk of liver metastasis occurs.MiRNA-27a-3p might participate in the occurrence and development of colorectal cancer by regulating target gene expression of GP73.In peripheral blood of colorectal cancer patients,miRNA-27a-3p might be used for potential auxiliary diagnosis and predict liver metastasis.