茶多酚提高心肺复苏大鼠脑组织过氧化物歧化酶表达和抑制细胞凋亡的研究

Tea polyphenols improves superoxide dimutase expression and inhibits apoptosis in brain tissue after cardiopulmonary resuscitation

目的探讨茶多酚（TP）对心肺复苏后大鼠脑组织过氧化物歧化酶（SOD）表达及细胞凋亡的影响。方法经食道交流电刺激诱导大鼠发生心室颤动（vF），建立心脏骤停/心肺复苏（CA／CPR）模型。假手术（SH）组只行手术操作，不诱导CA／CPR（n=6）。恢复自主循环的48只大鼠随机分成两组，分别为TP组（10mg／kg，iv．，n=24）和模型组（注射等量生理盐水，n=24），每组分别设12h、24h、48h、72h四个检测时间点，每个时间点有6只大鼠。用免疫荧光标记法检测大脑皮层SOD的荧光密度，Caspase3／TUNEL双标记法检测细胞凋亡。结果CPR后，模型组和TP组在12h、24h、48h的SOD荧光密度均较假手术组明显降低（P〈0．05）；TP组在四个时间点的SOD荧光密度均较模型组明显升高（P〈0．05）。CPR后72h，TP组的Caspase3／TUNEL阳性率较模型组明显降低（P〈0．05）。结论茶多酚能提高心脏骤停／心肺复苏后大鼠脑组织SOD的表达水平，抑制细胞凋亡。

Objective This essay is written to explore whether tea polyphenols （TP） improves the expression of superoxide dimutase and inhibits cell apoptosis in brain tissue after cardiopulmonary resuscitation （CPR）. Methods Cardiac arrest/cardiopulmonary resuscitation （CA/CPR） model was established using the method of alternating current-esophagus stimulation. Sham group （SH） was treated without CA/CPR （n = 6）. Forty-eight rats, once spontaneous circulation restoring, were divided into two groups, tea TP group （ 10 mg/kg, iv. , n = 24） and model group （injection equal of normal saline, n = 24）. Each group respectively was set 12 h, 24 h, 48 h, 72 h four detection points, with 6 rats in each time point. The cerebral tissue of superoxide dismutase fluorescence density and apoptotic cells were detected with immunofluorescence labeling method and Caspase3/TUNEL double labeling method. Results After CPR, the SOD fluorescence density of both model and TP group was lower than that of control group （P 〈 0.05）. The density in TP group was significantly higher than that in the model group at four time points （P 〈 0.05）. 72 h after CPR, Caspase3/TUNEL positive rate of TP group was obviously lower in the model group （P 〈 0.05 ）. Conclusion Tea polyphenol can improve the SOD expression and inhibit cell apoptosis in brain tissues of rats after CPR.