香菇菌丝体多糖的化学结构与抗氧化活性分析

Characteristic and antioxidant activities of polysaccharides by Lentinus edodes mycelium

对香菇菌丝体多糖(Lentinus edodes mycelium polysaccharides,LMPS)的2种组分(LMPS-1和LMPS-2)进行化学结构特征分析,旨为香菇菌丝体多糖的构效关系研究提供依据。文中利用高效液相色谱法(HPLC)、气相色谱(GC)、红外光谱(IR)分析等对其化学结构特征进行解析,并测定其抗氧化活性。构成糖分析结果显示:LMPS-1单糖组成为阿拉伯糖、木糖、甘露糖和葡萄糖,其摩尔比为2.5∶0.8∶1∶1.8;LMPS-2的单糖组成为鼠李糖、木糖和葡萄糖,其摩尔比为4.2∶1∶2.7。LMPS-1和LMPS-2均有较强的抗氧化活性,LMPS-2更为显著。香菇菌丝体多糖主要由阿拉伯糖和鼠李糖组成的吡喃型多糖,有较强的抗氧化活性。

To isolate and purify Lentinus edodes mycelium polysaccharides （LMPS） through ion-exchange chro- matography and gel filtration chromatography, and to provide a theoretical reference for relationship between structure and activity of LMPS. The structural characteristics of polysaccharide were elucidated by High Performance Liquid Chromatography （HPLC）, gas chromatography （GC）, and infrared spectrum （IR）. Results： The result showed that LMPS-1 was composed of rhamnose （Rha） , arabinose （Ara） , xylose （Xyl）, mannose （Man）, glucose （Glu） and galactose （Gal） with a molar ratio of 1.52：2.96：2.91：0.78 ： 1 ： 1.35 ; LMPS-2 contained Rha, Ara and Xyl with a molar ratio of 2.91 ： 1 ： 1.1：0.2：0.5.. The results showed that LMPS-1 and LMPS-2 had very strong reducing power and scavenging effects on DPPH and hydroxyl radicals. LMPS furanoids were mainly composed of Rha and Glu with strong antioxidant activities.