两种吸虫复染制片技术的探究

Exploration on the Technology of Counter Staining of Fluke

采用醋酸卡红溶液和孔雀绿水溶液,对固定后的华支睾吸虫和布氏姜片虫标本进行复合染色。醋酸卡红对两种吸虫标本进行单染。复染法使得标本虫体呈三色,消化系统、排泄系统和周围肌肉组织均为浅红色,生殖器官中的子宫呈现鲜绿色,虫体两侧卵黄腺为黄褐色,虫体结构清晰、着色适度、立体感强。醋酸卡红染液单染对吸虫睾丸组织的显现力更强。在吸虫的教学和科研工作中,结合使用单染法和复染法,观察效果将会更好。

Fixed samples of Clonorchis sinensis and Fasciolopsis buski were stained with acetocarmine and malachite green, or stained with acetocarmine only. The samples displayed three different colors after staining with acetocarmine and malachite green. The digestive system, excretory system and the surrounding muscle tissue were stained reddish, the uterus was bright green, and the vitellarium at each side of the worm was tan. Staining with the two dyes resulted in clear structure and moderate degree of staining, and allowed three-dimensional observation, while staining with acetocarmine highlighted the testis tissue. Therefore, combination of the two staining methods is recommended in teaching and research to more effectively facilitate observation.