贵州省抑郁症患者刚地弓形虫感染状况调查及其基因型鉴定

Survey on Toxoplasma gondii Infection in Depressed Patients and Genotype Identification in Guizhou Province

目的 了解贵州省抑郁症患者弓形虫感染状况及弓形虫基因型。 方法 ELISA检测141例抑郁症患者和150例健康对照外周血弓形虫特异性抗体（IgG、IgM）和循环抗原（CAg），PCR扩增血样弓形虫高重复DNA片段（529 bp），多重-巢式PCR-限制性片段长度多态性（Mn-PCR-RFLP）方法鉴定弓形虫529 bp阳性样本的基因型。 结果 ELISA检测结果显示，抑郁症患者组和健康对照组弓形虫抗体阳性率分别为21.3%（30/141）和7.3%（11/150），两者间差异有统计学意义（χ2=11.674，P〈0.05）；抑郁症患者组的IgG、IgM、CAg阳性率分别为18.4%（26/141）、1.4%（2/141）、1.4%（2/141）；健康对照组IgG阳性率为7.3%（11/150），与抑郁症患者组间差异有统计学意义（P〈0.05），未检测到IgM和CAg。PCR结果显示，抑郁症患者组检测出1例弓形虫核酸阳性，经Mn-PCR-RFLP方法鉴定其基因型，结果为非典型Toxo DB #9（Chinese 1型）。 结论 贵州省抑郁症患者弓形虫抗体阳性率高于健康人群，1例抑郁症患者感染的弓形虫基因型为Chinese 1型。

Objective To survey on Toxoplasma gondii infection in depressed patients in Guizhou Province and identify the genotype of T. gondii. Methods Enzyme-linked immunosorbent assay（ELISA） was applied to detect the T. gondii-specific antibodies IgG, IgM and circulating antigens （CAg） of T. gondii in 141 patients and 150 healthy subjects. The specific repeated DNA fragment（529 bp） of T. gondii was amplified by PCR. The genotype of T. gondii was determined by multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism （Mn-PCR-RFLP）. Results ELISA showed that the positive rate of anti-T. gondii antibody in depressed patients and healthy subjects was 21.3%（30/141） and 7.3%（11/150）, respectively. The positive rate of IgG in depressed patients was 18.4% （26/141）, significantly higher than that in healthy subjects （7.3%, 11/150）（P〈0.05）. The positive rate of IgM and CAg in depressed patients was both 1.4% （2/141）, while these were not found in healthy subjects. PCR revealed one patient positive for T. gondii, whose genotype was further identified to be Toxo DB #9（Chinese 1 type） by Mn-PCR-RFLP. Conclusion The positive rate of T. gondii is higher in depressed patients than in the healthy subjects in Guizhou Province. The genotype of T. gondii detected in one depressed patient is the Chinese 1 type.