摘要
目的比较醇提工艺和水煎工艺生产的蠲痹胶囊(熟地黄、肉苁蓉、骨碎补等)防治大鼠膝骨性关节炎的差异。方法 90只SD大鼠,除空白组10只外,其余采用4%木瓜蛋白酶制备膝骨性关节炎模型,随机分为模型组,壮骨关节丸组,水煎工艺和醇提工艺蠲痹胶囊的低、中、高剂量组。给药30 d后,各组大鼠称重,检测血清及关节腔液中环氧化酶-2(COX-2)、一氧化氮(NO)、诱导型一氧化氮合酶(i NOS)水平;病理学检查取各组胫骨平台关节软骨,分别进行HE、AB-PAS染色及肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)免疫组化染色,观察各组关节软骨形态及炎性因子表达。结果醇提工艺蠲痹胶囊比水煎工艺更能显著降低膝骨性关节炎和大鼠血清及关节液中COX-2、NO、i NOS及TNF-α、IL-1β含有量,延缓软骨降解。结论蠲痹胶囊醇提工艺取代水煎工艺是合理的。
AIM To compare the effects between the water and the alcohol extraction of Juanbi Capsules （Re- hmanniae Radix Praeparata, Cistanches Herba, Drynariae Rhizoma, etc. ） in treating rats with knee osteoarthritis. METHODS Eighty rat modelled for knee osteoarthritis was induced by 4% papain and then were randomly divid- ed into model group, Zhuanggu Guanjie Pills group, water-extraction Juanbi Capsules low, medium and high dose groups, alcohol-extraction Juanbi Capsules low, medium and high dose groups. Another ten rats served as the blank group. After consective intragastric administration for thirty days, rats were weighed. The levels of COX-2, NO and iNOS in serum and knee joint cavity fluid were detected. Tibial articular cartilage was taken for pathologi- cal examination. HE, AB-PAS staining and TNF-α and IL-1 β immunohistochemical staining were performed to ob- serve the histomorphology of articular cartilage and expressions of inflammatory factor. RESULTS Alcohol-ex- traction Juanbi Capsules were superb to the water-extraction Juanbi Capsules in reducing knee osteoarthritis and lev- els of COX-2, NO and iNOS in serum and joint flouid, and slowing down cartilage degradation. CONCLUSION Alcohol extraction is more suitable to Juanbi Capsules than water-extraction.
出处
《中成药》
CAS
CSCD
北大核心
2016年第8期1661-1668,共8页
Chinese Traditional Patent Medicine
基金
陕西中医学院中西医结合防治骨退行性疾病研究创新团队(2013KCT-26)
