沉香GC-MS指纹图谱分析

Analysis of Aquilaria sinensis GC-MS fingerprints

目的建立沉香Aquilaria sinensis（Lour.）Gilg的GC-MS指纹图谱,并进行分析。方法分析采用HP-5MS色谱柱;载气He;进样量1.0μL;离子源温度230℃;四极杆温度150℃;质量扫描范围m/z 29~500。结果 10批样品中检测到90个色谱峰,鉴定出75种成分,其中共有成分11种。GC-MS指纹图谱可分成倍半萜类区间和色酮类区间,相似度0.48~0.92。R2为0.67时,样品可分为2类;R2为0.82时,样品可分为3类。利用建立的指纹图谱对2批整树结香法所产的沉香和2批未知样品进行分析。发现2批整树结香法所产沉香中化合物的类型更接近于黄熟香,色酮类成分含有量明显高于倍半萜类成分。2批未知样品分别加入了邻苯二甲酸二（2-乙基己）酯和沉香提取物。结论GC-MS指纹图谱能快速检测沉香中挥发性成分,并可用于真伪鉴别。

AIM To establish the GC-MS fingerprints of Aquilaria sinensis （ Lour. ） Gilg and to make analysis. METHODS The analysis was performed on HP-5MS column, carrier gas was He, injection volume was 1 μL, ion source temperature was maintained at 230 ℃, quadrupole temperature was maintained at 150 ℃, and mass scan range was m/z 29 - 500. RESULTS Ninety chromatographic peaks were detected in ten batches of samples, and seventy-five constituents were identified, eleven of which were common constituents. The GC-MS fingerprints can be divided into sesquiterpene area and chromone area, whose similarities were 0. 48 - 0.92. When R2 was 0. 67, the samples could be divide into two types. When R2 was 0. 82, the samples could be divide into three types. Two artificial agarwood which produced by whole-tree agarwood-inducing technology and two unknow sam- ples were analyzed by the fingerprint. It found that the types of constituents in two batches of A. sinensis produced by whole-tree agarwood-inducing technique were more similar to those in Huangshuxiang, and the contents of chromones were much higher than those of sesquiterpenes. Two batches of samples were added with dioctyl phthalate and A. Sinensis extract, respectively. CONCLUSION GC-MS fingerprints can rapidly detect the vola- tile constituents in A. sinensis, which can be also used for the identification.