太阴病脾阳虚证患者唾液分泌型IgA含量变化及舌苔蛋白双向电泳图谱研究

Content Variance of Secretory IgA in Patients of Spleen-Yang Deficiency Syndrome and Study on Two-dimensional Electrophorogram of Coated Tongue Protein

目的:探讨脾阳虚证和正常人口腔唾液分泌型Ig A含量变化及舌苔蛋白SDS-PAGE电泳图谱差异,进而从免疫及蛋白表达方面揭示脾阳虚证的特征变化规律。方法:参照《中药新药临床指导原则》的临床实施标准,将确诊的脾阳虚证患者及正常人,纳入本实验。采用酶联免疫吸附测定法(ELISA)测定分泌型Ig A含量及采用蛋白双向电泳方法对舌苔蛋白电泳图谱进行差异分析。结果:分泌型Ig A含量与正常人比较有显著差异(P<0.05);而舌苔蛋白双向电泳图谱与正常人比较,两组间存在21个具有明显表达差异的蛋白斑点。结论:脾阳虚证患者口腔免疫水平显著下降是脾阳虚证的基本特征之一。脾阳虚证患者和正常人舌苔蛋白表达确实存在差异。

Objective： Discussing the content variance of secretory Ig A in saliva between spleen-Yang deficiency syndrome patients and healthy persons,and then open out the characteristic variation law of spleen-Yang deficiency syndrome from immune and protein expression. Methods： Referring to the authorized literature of diagnostic standard to spleen- Yang deficiency syndrome,bringing into the patients who had been diagnosed. Adopting the method of ELISA to determine the content of secretory Ig A and adopting protein two-dimensional electrophoresis method to exert variance analysis of electrophoretogram of coated tongue protein. Results： There existed significant difference between the two groups（ P0. 05）; there existed 21protein？ spots that had obvious expression difference between the two groups. Conclusion： The obvious decline of mouth immune level is one of the basic feature of spleen-Yang deficiency syndrome. Difference of coated tongue protein expression between spleen-Yang deficiency patients and normal person certainly exist.