艾滋病热毒蕴结证蛋白组学标志物验证研究

Proteomics markers verification studies of AIDS Heat-toxin Stagnation Syndrome

目的:通过Western Blot技术对显著性差异蛋白进一步临床验证,为艾滋病热毒蕴结证具有诊断及疗效评价意义的蛋白分子标志物研究提供实验依据。方法:本研究于前期i TRAQ技术研究基础上,采用蛋白质印迹法(Western Blot)技术分别对采集的AIDS热毒蕴结证患者和健康对照组血清标本进行差异蛋白检测,运用image J软件分析灰度值,然后将每种抗体中的正常组的相对灰度值和患者组的相对灰度值导入到Graph Pad Prism 5软件,得出mean+/-SD的图表。结果:艾滋病期热毒蕴结证组与正常对照组比较,C5在艾滋病期热毒蕴结证表达下调,差异有统计学意义(P<0.05),与i TRAQ筛选结果一致。结论:获得的艾滋病期热毒蕴结证特征性表达蛋白C5,为建立艾滋病中医证候诊断模式,完善艾滋病辨证规范和辨证方法奠定基础。

Objective： through Western Blot technique for further clinical verification,significant differences in protein for AIDS toxic heat wenjie syndrome diagnosis and curative effect evaluation of significance of protein molecular markers to provide experimental basis. Method： this research in the early stage of the i TRAQ technology research,on the basis of using Western Blot method（ Western Blot） technology to sampled respectively AIDS toxic heat wenjie syndrome patients and healthy control group differences in protein detection serum samples,using the gray levels image J software analysis,and then the relative grey value of each antibody in the normal group and patient group the relative grey value of imported into the Graph Pad Prism 5 software,it is concluded that the mean ＋ /- SD charts. Results： the AIDS issue toxic heat wenjie card group compared with normal control group,C5 in the AIDS issue toxic heat wenjie,the expression difference was statistically significant（ P 0. 05）,consistent with i TRAQ screening results. Conclusion： this study AIDS period toxic heat wenjie certificate validation C5 characteristic expression of protein,to establish a pattern of syndromes of traditional Chinese medicine AIDS,laying a foundation for perfect AIDS syndrome differentiation standard and dialectical method.