摘要
本研究的目的是克隆广西巴马小型猪SLC30-A8基因,并确定各组织的表达量。利用RT-PCR的方法扩增并克隆SLC30-A8基因;荧光定量PCR检测SLC30-A8基因在12月龄广西巴马小型猪心脏、肝脏、脾脏、肺脏、肾脏、胰腺、小肠和脂肪中的表达。结果显示,成功克隆广西巴马小型猪SLC30-A8基因CDS区全长1 110 bp,荧光定量PCR结果显示SLC30-A8基因在12月龄广西巴马小型猪胰腺组织中表达量最高,其次是心脏、脾脏,在肺脏、小肠以及脂肪组织中几乎不表达。本研究成功克隆了广西巴马小型猪SLC30-A8基因并确定在胰腺组织中表达最高。该研究将为下一步研究SLC30-A8基因在糖尿病的发生过程中对糖代谢的作用奠定基础。
The purpose of this study is to clone Guangxi Bama mini-pig SLC30-A 8 gene, and to determine the expression level of the tissues. Using the method of RT-PCR to amplificate and clone SLC30-A 8 gene; fluor- escence quantitative PCR to detect the expression of SLC30-A8 gene in the heart, liver, spleen, lung, kidney, pancreas, small intestine and fat of Guangxi Bama mini-pig in 12 months of age. The results showed that the full length CDS 1 110 bp of Guangxi Bama mini-pig SLC30-A8 gene was cloned successfully. The results of fluor- escent quantitative PCR showed there were the highest expression of SLC30-A8 gene in pancreas of Guangxi Bama mini-pig in 12 months of age, followed by the heart and spleen, and almost no expression in lung, fat and small intestine. The study lays the foundation for further study of the SLC30-A8 gene in effects on glucose metabolism in the process of diabetes.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2016年第8期1935-1938,共4页
Genomics and Applied Biology
基金
广西科技基础条件平台建设项目不同诱导型广西巴马小型猪2型糖尿病动物模型转录组的研究(14-91-07)
国家现代农业产业技术体系广西生猪创新团队项目(nycytxgxcxtd-03-15)共同资助
