LAMA4在胎盘组织中的差异表达及其在滋养细胞中的功能

Expression and function of LAMA4 in the placentas and trophoblast

目的:探讨层粘连蛋白α4链(laminin alpha4,LAMA4)在胎盘组织中的差异表达及其对滋养细胞功能的影响,揭示其在子痫前期(preeclampsia,PE)发病机制中的作用。方法:收集22例PE和20例正常产妇的胎盘组织,应用免疫组化方法检测LAMA4蛋白在胎盘组织中的表达。采用RNA干扰技术,将滋养细胞分为正常培养对照组、缺氧/复氧(hypoxia/reoxygenation,H/R)培养组及干扰培养组,采用细胞免疫荧光和Western blot法检测LAMA4蛋白在各组细胞中的表达。采用Transwell小室模型检测各培养组细胞的侵袭功能。结果:(1)子痫前期胎盘组织中LAMA4蛋白的表达水平明显低于对照组(P<0.05)。(2)H/R及干扰处理后滋养细胞中LAMA4蛋白的表达水平明显降低(P<0.05)。(3)下调LAMA4的蛋白表达后滋养细胞的侵袭率明显降低(P<0.05)。结论:LAMA4蛋白表达的下调可抑制滋养细胞的侵袭功能,可能参与子痫前期的发病。

Objective：To investigate the expression of the laminin alpha4（LAMA4 ） gene and its effect on invasion of HTR-8/SVneo cells and its role in the pathogenesis of preeclampsia. Methods.Twenty-two patients with severe pre-eclampsia and 20 normal preg- nant women were investigated. The expression of LAMA4 was determined by immunohistochemistry. Using RNA interference technol- ogy,the trophoblast cells were divided into the normal control group,hypoxia/reoxygenation（H/R） oxygen culture group and LAMA4 interference group. Expression of lama4 in each group was detected by the immune fluorescence and Western blot. The invasive ability of the HTR-8/SVneo cells was tested by transwell. Results： （ 1 ）The expression of LAMA4 in severe pre-eclampsia group was dramati- cally lower than that of control group. （2）The expression of LAMA4 in HTR-8/SVneo cells was significantly decreased after H/R and siRNA treatments. （3）The invasive ability of HTR-8/SVneo cells was deduced after the deduction of LAMAg expression. Conclusion： Damage of LAMA4 is probably involved in the mechanism of onset of preeclampsia by regulating invasion of trophoblast.