不同干燥方式对百蕊草中两种黄酮醇苷含量影响

Effect of Different Drying Methods on the Two Flavonol Glycosides Content in Thesium chinense Turcz

研究不同干燥方法对百蕊草及百蕊草不同部位中槲皮素醇苷和山柰素醇苷含量的影响和百蕊草不同部位中两种黄酮醇苷的含量分布。采用阴干、晒干、105℃杀青后80℃烘干、霉变后80℃烘干4种干燥方式及80℃,60℃,40℃直接烘干3种不同烘干温度处理百蕊草样,并用80℃烘干百蕊草叶、茎、叶、果实、根不同部位样,测定其酸水解液中槲皮素及山柰素含量,计算其两种黄酮醇苷含量。4种不同干燥方式对百蕊草中两种黄酮醇苷含量影响为烘干=晒干>阴干>酶变,3种不同烘干温度对百蕊草中两种黄酮醇苷含量影响为:40℃烘干样<80℃烘干样=60℃烘干样。百蕊草不同部位样中山奈素醇苷分布为叶>茎>种子>根;而其槲皮素醇苷分布由高至低则为:茎>叶>种子>根。明确了百蕊草根、茎、叶及种子中槲皮素及山柰素醇苷的分布,其中叶中含量最高,同时确定百蕊草最佳干燥方式为烘干,且80℃及60℃烘干温度均能有效地保持百蕊草中两种黄酮醇苷成分。

Our purpose is to study on the influence of different drying methods to kaempferol glycosides contents and quercetin glycosides contents of Thesium chinense Turcz and the distribution of two kinds of flavone glycosides contents in different parts of T. chinense. T. chinense was dried by four different drying methods of drying in the shade, drying in the sun, drying at 80℃ after fixing at 105℃ and drying at 80℃ after mildew, and at three different temperatures of 80℃, 60℃, 40℃. The root, stalk, leaf and seed of T. chinense were dried in 80℃. Kaempferol glycoside and quercetin glycoside contents were indentified with HPLC. The order of influence of four different drying methods to kaempferol glycoside and quercetin glycoside contents was drying over a fire=drying in the sun 〉 drying in the shade 〉 mildewed. The order of influence of three different drying temperature to Kaempferol glycoside and quercetin glycoside contents was 40℃〈80℃=60℃. The distribution order of kaempferol glycosides contents in different parts of T. chinense was leaf 〉 stalk 〉 seed 〉 root. The distribution order of quercetin glycosides contents in different parts of T. chinense was stalk 〉 leaf 〉 seed 〉 root. The two kinds of flavone glycosides content in the leaves of T. chinense was the highest. It is concluded that the best drying method is drying and the best drying tempreture is 80℃ and 60℃.