艾渣中有效成分的定性检测及含量测定

Study on the Qualitative Test and Detection Method of Active Components in Slag of Blumea balsamifera Extraction

为给艾渣资源化回收利用提供技术依据,通过显色定性试验和泡沫试验检测艾渣中有效成分,并采用UV法建立艾渣中有效成分含量的测定方法。结果显示:艾渣中含有鞣质、蒽醌类化合物、黄酮类化合物、多糖和皂苷类化合物等多种物质,无生物碱;试验采用UV法,以大黄素、芦丁、绿原酸、人参皂苷Rg1、葡萄糖为对照,在222、420、327、554、620 nm处测定艾渣中总蒽醌3.176、总黄酮12.853、总有机酸18.715、总皂苷9.512和总多糖13.120 mg/g。方法学研究表明:各有效成分测定方法线性关系良好、精密度高、回收率高、重复性好、稳定性强。综上所述,艾渣提取蒽醌类、黄酮类、皂苷类和多糖化合物等物质具有较高的回收利用价值。

In order to provide reliable basis for recycling and utilization of slag of Blumea extraction, the active components were detected by qualitative test and foam test, and the method for determination of the content of active components in slag of Blumea extraction were established by using UV method. The results indicated that the slag of Blumea extraction contained tannins, anthraquinones, flavonoids, polysaccharides and saponins, no alkaloids. Emodin, rutin, chlorogenic acid, ginsenoside （Rg1） and glucose were used as the reference substance, the contents of total anthraquinones, total flavonoids, total organic acids, total saponins and total polysaccharides were measured in 222, 420, 327, 554, 620nm, they could reach 3.176, 12.853, 18.715, 9.512, 13.120mg/g, respectively. These five proposed methods have precise, high precision and recovery, good repeatability and stability, which are simple, sensitive, accurate and reproducible. It is concluded that the way have high recovery utilization value for using slag of Blumea extraction to extract anthraquinones, flavonoids, polysaccharides, saponins and so on.