超疏水釉质表面抑制黏蛋白吸附和细菌黏附

The superhydrophobic enamel surface inhibition of mucin adsorption and bacterialadhesion

目的探讨涂有超疏水材料釉质片表面对于黏蛋白吸附和变形链球菌黏附的影响。方法制备牛牙釉质块若干,随机分为两组:用十七氟癸基三甲氧基硅烷处理釉质片作为实验组,用去离子水处理牙釉质片作为空白对照组。用场发射扫描电镜对样品分别面进行检测。对两组样品分别进行接触角和唾液黏蛋白的吸附的测定。通过场发射扫描电子显微镜观察样品表面生物膜形态特征以及计数菌落形成单位评估样品表面变形链球菌生物膜形成情况。结果场发射扫描电镜检测样品表面可见实验组与对照组形貌不同。接触角测试结果显示,实验组的接触角明显大于对照组的接触角(P<0.05)。实验组样品的唾液黏蛋白的吸附量明显少于对照组(P<0.05)。场发射扫描电镜观察结果显示2 h和24 h的实验组样品表面均只有极少量的菌落,而对照组样品表面有大量的菌斑生物膜。48 h后,两组样品表面均被大量细菌生物膜完全覆盖。菌落形成单位计数结果与扫描电镜结果一致。结论涂有超疏水材料釉质片表面对于黏蛋白吸附和变形链球菌生物膜形成有抑制作用。

Objective To investigate the effect of superhydrophobic enamel surfacein salivary mucin adhesion and oral biofilm formation. Methods Bovine enamel blocks were prepared as specimens and they were randomly divided into two groups. The experimental group received（ heptadecafluoro-1,1,2,2-tetradecyl） trimethoxysilane application,while the control group received deionized water application. The sample surfaces were detected by field emission scanning electron microscope（ FE-SEM）. Contact angles and salivary mucin absorption were measured.The morphological characteristics were observed by FE-SEM and oral biofilm formation inhibition on enamel disks was assessed by counting colony-forming units（ CFU）. Results The surface topographies of the experimental group and the control group were different according to the FE-SEM. And the contact angles of the experimental group were significantly bigger than the control group（ P 〈0. 05）. The mucin absorbed by the experimental group was significantly less than the control group（ P 〈0. 05）. Very few bacteria were found in the experimental group after 4hours and 24 hours. While bacteria were widely distributed in the control group. After 48 hours bacteria growth,the surfaces were completely covered by biofilms in all samples. And the results of Log（ CFU） were consistent with that. Conclusion Superhydrophobic surfaces can inhibit salivary mucin adhesion and oral biofilm formation.