原花青素对大鼠缺血再灌注损伤脑组织中超氧化物歧化酶、谷胱甘肽过氧化物酶、丙二醛和过氧化脂质水平的影响

Effect of proanthocyanidin on the levels of superoxide dismutase,glutathione peroxidase,malondialdehyde and lipid peroxides in brain tissue of rats with cerebral ischemia reperfusion injury

目的探讨原花青素对大鼠缺血再灌注损伤脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)和过氧化脂质(LPO)水平的影响。方法 64只健康雄性Sprague-Dawley大鼠随机分为假手术组、模型组、原花青素高剂量(160 mg·kg-1)组和原花青素低剂量(80 mg·kg-1)组,每组16只。采用改良的ZeaLonga线栓法制备大鼠局灶性脑缺血再灌注损伤模型,并对每组大鼠进行相应的干预处理;比较4组大鼠脑组织中SOD、GSH-Px、MDA和LPO水平。结果与假手术组比较,模型组大鼠脑组织中SOD、GSH-Px水平显著降低(P<0.05),而MDA、LPO水平显著升高(P<0.05)。与模型组比较,原花青素高、低剂量组大鼠脑组织中SOD、GSH-Px水平显著升高(P<0.05),而MDA、LPO水平显著降低(P<0.05)。原花青素高剂量组大鼠脑组织中SOD、GSH-Px水平显著高于原花青素低剂量组(P<0.05),而MDA、LPO水平显著低于原花青素低剂量组(P<0.05)。结论原花青素可能通过其抗氧化作用对大鼠脑组织缺血再灌注损伤起到保护作用。

Objective To investigate the effect of proanthocyanidin on the levels of superoxide dismutase（SOD） ,gluta- thione peroxidase （GSH-Px）, malondialdehyde （MDA） and lipid peroxides （LPO） in brain tissue of rats with cerebral ische- mia reperfusion injury. Methods Sixty-four healthy male Spragne-Dawley rats were randomly divided into sham operation group, model group, high-dose proanthocyanidin group （160 mg · kg-1） and low-dose proanthocyanidin group （80 mg · kg-1） ,16 rats in each group. The models of focal cerebral ischemia reperfusion injury in rats were established by modified Zea-Longa suture method. The rats in the four groups were treated with corresponding intervention. The levels of SOD, GSH-Px, MDA and LPO in brain tissue of rats were detected in the four groups. Results The SOD and GSH-Px levels in brain tissue of rats in model group were significantly lower than those in sham operation group （ P 〈 0.05 ）, but the MDA and LPO levels in brain tissue of rats in model group were significantly higher than those in sham operation group （ P 〈 0.05 ）. The SOD and GSH-Px levels in brain tissue of rats in high- and low-dose proanthocyanidin group were significantly higher than those in model group（P 〈 0.05 ）, but the MDA and LPO levels in brain tissue of rats in high- and low-dose proanthocyanidin group were significantly lower than those in model group（ P 〈 0.05 ）. The SOD and GSH-Px levels in brain tissue of rats in high-dose proanthocyanidin group were significantly higher than those in low-dose proanthocyanidin group（P 〈 0.05）, but the MDA and LPO levels in brain tissue of rats in high-dose proanthocyanidin group were significantly lower than those in low-dose proantho- cyanidin group（P 〈 0.05）. Conclusion The antioxidant effect of proanthocyanidin may play a protective role in cerebral is- chemia reperfusion injury in rats.