山东省46个花生品种SSR指纹图谱构建与遗传多样性分析

Construction of Fingerprinting and Analysis of Genetic Diversity With SSR Markers for Forty-Six Approved Peanut Cultivars From Shandong Province

为从分子水平上快速鉴别花生品种和选配优良杂交组合,以山东省审定的46个花生品种为材料,利用微卫星（SSR）标记进行DNA指纹图谱的构建和遗传多样性分析。从788对SSR引物中筛选出50对多态性高、稳定性好、谱带清晰的引物,共检测到175个等位位点,其中122个为多态性位点,多态性比率达70.52%;每对SSR引物扩增出的等位位点数为2~7个,多态性信息量变化范围为0.6753~0.8412,平均为0.823。此外,利用14对引物可将46份材料完全区分开。聚类分析表明,在相似系数0.77处,所有供试材料聚为一类,在相似系数0.80处,仍有76%的材料聚在一起。利用SSR标记构建的指纹图谱可为花生种质资源管理及育种实践提供依据。

In this study,46 peanut cultivars of Shandong province were used as materials in construction of DNA fingerprinting and analysis of genetic diversity with simple sequence repeat（ SSR） markers. 50 of 788 pairs of SSR primers with high polymerphism,stability and clear bands were screened out. The 50 primer pairs amplified a total of175 alleles（ including 122 polymorphic alleles） among the 46 cultivars,and the ratio of polymorphism was as high as70. 52%. Alleles amplified by each pair of primers ranged from 2 to7. The polymorphic information content values（ PIC） ranged from0. 6753 to 0. 8412,with a mean of 0. 823. These 46 peanut cultivars could be absolutely identified with only 14 primer pairs. UPGMA cluster analysis of genetic similarity showed that all the materials were clustered in to one group at the genetic similarity of 0. 77,and 76% of the cultivars were still clustered together at the genetic similarity of 0. 80. The genetic relationships of cultivars were identical to the family tree basically. It is indicated that the genetic basis of peanut cultivars in Shandong province is narrow. Construction of fingerprinting with SSR markers will provide the basis for peanut germplasm resources management and breeding.