结直肠癌患者组织和外周血中细胞黏附分子-1和血管细胞黏附分子-1的表达及临床意义

Expression and clinical significance of ICAM-1 and VCAM-1 in colorectal cancer

目的检测结直肠癌(colorectal cancer,CRC)患者组织和外周血中细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)的表达,探讨ICAM-1和VCAM-1的临床意义。方法应用免疫组化SP法测定CRC原发病灶(68例)、非癌正常组织(68例)、结直肠腺瘤组织51例中的ICAM-1和VCAM-1的表达。应用酶联免疫吸附试验(ELISA)检测CRC患者(68例)和健康者(68例)血清中ICAM-1和VCAM-1的含量。结果 CRC原发病灶组织中,ICAM-1和VCAM-1明显高于非癌正常组织及结直肠腺瘤组织,差异有统计学意义(P<0.05)。CRC患者血清中ICAM-1和VCAM-1含量明显高于健康对照组,差异有统计学意义(P<0.05)。ICAM-1和VCAM-1的表达与性别、年龄、肿瘤部位和病理学分级无关,差异无统计学意义(P>0.05);但与淋巴结转移及Dukes分期相关,差异有统计学意义(P<0.05)。结论 CRC患者组织及外周血中ICAM-1和VCAM-1在CRC的发病过程中具有重要作用,对肿瘤的诊断、恶性程度判断和检测病情变化具有重要意义。

Objective To detect colorectal cancer（ CRC） tissue and peripheral blood in patients with intercellular adhesion molecule 1（ ICAM-1） and vascular cell adhesion molecule 1（ VCAM-1） expression and analyze the clinical significance of ICAM-1 and VCAM-1. Methods The immune histochemical method（ SP method）,68 cases of normal tissue and the carcinoma of CRC primary lesion,51 cases of the tissue of colorectal adenomas the expression of ICAM-1 and VCAM-1,application of ELISA to detect ICAM-1 and VCAM-1 serum content in cases of 68 CRC patients and 68 cases of healthy subjects. Results The CRC tissues from the primary lesion of VCAM-1 and ICAM-1 is significantly higher than the normal tissue and colorectal adenomas group（ P〈0. 05）,statistically significant difference; CRC patients serum ICAM-1 and VCAM-1 content is significantly higher than healthy controls（ P〈0. 05）,statistically significant difference; the expression of ICAM-1 and VCAM-1 is unrelated with gender,age,tumor location and pathological grading（ P〉0. 05）; But associated with lymph node metastasis and Dukes staging（ P〈0. 05）. Conclusion Patients with CRC tissues and peripheral blood ICAM-1 and VCAM-1 plays an important role in the process of the onset of CRC,and it is of great significance in the diagnosis of tumors,judgment of malignant degree and test of condition changes.