摘要
目的检测结直肠癌(colorectal cancer,CRC)患者组织和外周血中细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)的表达,探讨ICAM-1和VCAM-1的临床意义。方法应用免疫组化SP法测定CRC原发病灶(68例)、非癌正常组织(68例)、结直肠腺瘤组织51例中的ICAM-1和VCAM-1的表达。应用酶联免疫吸附试验(ELISA)检测CRC患者(68例)和健康者(68例)血清中ICAM-1和VCAM-1的含量。结果 CRC原发病灶组织中,ICAM-1和VCAM-1明显高于非癌正常组织及结直肠腺瘤组织,差异有统计学意义(P<0.05)。CRC患者血清中ICAM-1和VCAM-1含量明显高于健康对照组,差异有统计学意义(P<0.05)。ICAM-1和VCAM-1的表达与性别、年龄、肿瘤部位和病理学分级无关,差异无统计学意义(P>0.05);但与淋巴结转移及Dukes分期相关,差异有统计学意义(P<0.05)。结论 CRC患者组织及外周血中ICAM-1和VCAM-1在CRC的发病过程中具有重要作用,对肿瘤的诊断、恶性程度判断和检测病情变化具有重要意义。
Objective To detect colorectal cancer( CRC) tissue and peripheral blood in patients with intercellular adhesion molecule 1( ICAM-1) and vascular cell adhesion molecule 1( VCAM-1) expression and analyze the clinical significance of ICAM-1 and VCAM-1. Methods The immune histochemical method( SP method),68 cases of normal tissue and the carcinoma of CRC primary lesion,51 cases of the tissue of colorectal adenomas the expression of ICAM-1 and VCAM-1,application of ELISA to detect ICAM-1 and VCAM-1 serum content in cases of 68 CRC patients and 68 cases of healthy subjects. Results The CRC tissues from the primary lesion of VCAM-1 and ICAM-1 is significantly higher than the normal tissue and colorectal adenomas group( P〈0. 05),statistically significant difference; CRC patients serum ICAM-1 and VCAM-1 content is significantly higher than healthy controls( P〈0. 05),statistically significant difference; the expression of ICAM-1 and VCAM-1 is unrelated with gender,age,tumor location and pathological grading( P〉0. 05); But associated with lymph node metastasis and Dukes staging( P〈0. 05). Conclusion Patients with CRC tissues and peripheral blood ICAM-1 and VCAM-1 plays an important role in the process of the onset of CRC,and it is of great significance in the diagnosis of tumors,judgment of malignant degree and test of condition changes.
出处
《中国肿瘤临床与康复》
2016年第8期903-905,共3页
Chinese Journal of Clinical Oncology and Rehabilitation