Cripto-1介导的上皮间质转化在卵巢浆液性腺癌形成中的作用

Effect by Cripto-1 mediated epithelial-mesenchymal transition in formation of ovarian serous adenocarcinoma

目的检测Cripto-1、E-钙粘蛋白(E-cadherin)、波形蛋白(Vimentin)在卵巢肿瘤中的表达,探讨卵巢浆液性腺癌的发生发展机制,以及Cripto-1介导的上皮间质转化(EMT)在其中的作用。方法采用免疫组化SP法检测Cripto-1、E-cadherin、Vimentin在60例卵巢浆液性腺癌、30例卵巢交界性浆液性肿瘤、20例卵巢浆液性腺瘤中的表达,并探讨其表达程度的相关性及与临床病理参数之间的关系。结果 Cripto-1在卵巢浆液性腺瘤中的表达20.0%低于浆液性腺癌76.7%和交界性浆液性肿瘤56.7%,差异具有统计学意义(P<0.05),E-cadherin在卵巢浆液性腺瘤中的表达90.0%高于浆液性腺癌35.0%和交界性浆液性肿瘤53.3%,差异具有统计学意义(P<0.05)。Cripto-1、Vimentin在卵巢浆液性腺癌中的表达与组织学分级、淋巴结转移有关(P<0.05);E-cadherin的表达与组织学分级有关(P<0.05),与淋巴结转移无关(P>0.05)。在卵巢浆液性腺癌中Cripto-1与E-cadherin的表达呈负相关(r=-0.81,P<0.05),Cripto-1与Vimentin的表达呈正相关(r=0.42,P<0.05)。结论在卵巢浆液性腺癌中Cripto-1、Vimentin表达上调,E-cadherin表达下调,提示Cripto-1介导的EMT可能在卵巢浆液性腺癌侵袭转移过程中起作用。

Objective To detect expression of Cripto-1, E-cadherin and Vimentin in ovarian tumor, and to investigate development mechanism of ovarian serous adenocarcinoma and effect by Cripto-1 mediated epithelial-mesenchymal transition（EMT）. Methods Immunohistochemical SP test was applied to detect expression of Cripto-1, E-cadherin and Vimentin in 60 cases with ovarian serous adenocarcinoma, 30 cases with ovarian borderline serous tumor and 20 cases with ovarian serous adenoma. Investigation was made on relationship between expression degree and clinical pathological parameters. Results Expression of Cripto-1 was lower in ovarian serous adenoma as 20.0% than 76.7% in ovarian serous adenocarcinoma and 56.7% in ovarian borderline serous tumor, their difference had statistical significance（P〈0.05）. Expression of E-cadherin was higher in ovarian serous adenoma as 90.0% than 35.0% in ovarian serous adenocarcinoma and 53.3% in ovarian borderline serous tumor, their difference had statistical significance（P〈0.05）. Expression of Cripto-1 and Vimentin showed their relationship with histological grade and lymphatic metastasis in ovarian serous adenocarcinoma（P〈0.05）; while expression of E-cadherin was related with histological grade（P〈0.05） and not related with lymphatic metastasis（P〈0.05）. Cripto-1 and E-cadherin showed negatively correlated expression in ovarian serous adenocarcinoma（r=-0.81, P〈0.05）, and expression of Cripto-1 and Vimentin showed their positive correlation（r=0.42, P〈0.05）. Conclusion Up-regulated expression of Cripto-1 and Vimentin and down-regulated expression of E-cadherin in ovarian serous adenocarcinoma suggest the effect by Cripto-1 mediated EMT in invasion and metastasis of ovarian serous adenocarcinoma.