新生儿坏死性小肠结肠炎早期诊断的生化指标检测

Detection of biochemical index for early diagnosis of neonatal necrotizing enterocolitis

目的探讨肠脂肪酸结合蛋白(I-FABP)表达水平对早期诊断新生儿坏死性小肠结肠炎(NEC)的意义。方法 58例NEC患儿作为实验组,58例非NEC患儿作为对照组,以酶联免疫吸附试验(ELISA)法测定患儿血清中I-FABP表达水平,并进行组间比较。结果实验组入院第1天Ⅰ、Ⅱ、Ⅲ级患儿血清中I-FABP水平(169.81±22.38)、(238.42±21.40)、(317.44±26.88)U/L及治疗第7天(52.01±7.81)、(58.23±8.88)、(68.83±8.89)U/L明显高于对照组(68.88±20.61)、(46.97±7.80)U/L(P<0.05);且随着级别越高,患儿I-FABP水平越高(P<0.05)。结论 I-FABP可作为早期诊断NEC较准确的生物学指标。

Objective To investigate significance by expression level of intestinal fatty acid binding protein（I-FABP） for early diagnosis of neonatal necrotizing enterocolitis（NEC）. Methods There were 58 infants with NEC as experimental group and 58 infants without NEC as control group. Enzyme-linked immuno sorbent assay（ELISA） was applied to detect expression level of serum I-FABP for comparison between the two groups. Results Infants in grades Ⅰ, Ⅱ, Ⅲ in the experimental group had serum I-FABP as（169.81±22.38）,（238.42±21.40）,（317.44±26.88） U/L in the 1st day of admission and（52.01±7.81）,（58.23±8.88）,（68.83±8.89） U/L in the 7th day of treatment, and those were obviously higher than（68.88±20.61） and（46.97±7.80） U/L in the control group（P〈0.05）. Higher grade led to higher I-FABP level in infant patients（P〈0.05）. Conclusion I-FABP can be taken as an accurate biochemical index for early diagnosis of NEC.