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HJURP基因表达下调对人胚胎绒毛细胞增殖凋亡作用的研究

Effect of silencing HJURP gene on proliferation and apoptosis of human embryonic villus cells
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摘要 目的研究HJURP基因的表达下调对人胚胎绒毛细胞增殖、细胞周期和细胞凋亡的影响。方法用CCK8法、Annexin V-EGFP/PI双染法、TUNEL法、Trans Well迁移侵袭实验等方法研究HJURP表达下调对人胚胎绒毛细胞增殖、细胞凋亡及细胞周期的影响。结果 HUJRP基因表达下调后,与对照组比较,明显抑制胚胎绒毛细胞的增殖及迁移侵袭能力。胚胎绒毛细胞的凋亡率为9.87±1.92%增加至,明显高于NS组3.92±1.12%(P<0.01)。对细胞周期的影响表现为G0/G1期阻滞,G0/G1期细胞从42.9±3.12%增加至62.4±1.92%。Trans Well侵袭实验结果显示,实验组穿过Trans Well膜的数目明显少于对照组。结论 HJURP基因的表达下调可以导致人胚胎绒毛细胞增殖抑制,诱导人胚胎绒毛细胞凋亡,使人胚胎绒毛细胞周期阻滞于G0/G1期,推测HJURP基因表达下调与人胚胎自然流产有相关性。 Objective：To study effect of silencing HJURP gene on proliferation,cycle and apoptosis of human embryonic villus cells,to study the possible mechanism of HJURP gene in spontaneous abortions. Methods：The effect of silencing HJURP gene were investigated by the methods of CCK8,Annexin V-EGFP / PI double staining,TUNEL method,Trans Well migration assay in human embryonic villus. Results：Compared with the control group,silencing HJURP gene significantly inhibited the activity of embryonic villus cells and cell cycle G2 / M phase,inhibited the proliferation and migration and invasion capacity of the cells. And TUNEL-positive rate was significantly higher,and improved the level of apoptosis. Conclusion：Silencing HJURP gene can induce apoptosis and cell cycle arreste in G0 / G1 phase,its action mechanism may be correlated with the occurrence of spontaneous abortion.
作者 薛慧琴 高庭 朱镭 孙夏瑜 卢洪涌 张玉萍 周岩 燕美琴 祁春茹
机构地区 山西省儿童医院遗传室山西省妇幼保健院
出处 《中国优生与遗传杂志》 2016年第8期31-34,共4页 Chinese Journal of Birth Health & Heredity
基金 山西省卫计委青年基金项目(编号:201201012)
关键词 HJURP基因 人胚胎绒毛细胞 凋亡 自然流产 HJURP gene Human embryonic villus cells Apoptosis Spontaneous abortion
分类号 R321 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

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中国优生与遗传杂志
2016年 第8期

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