子痫前期患者胎盘、胎膜组织中水通道蛋白9的表达变化

Expression of aquaporin-9 in placenta and fetal membrane of preeclampsia patients

目的：观察水通道蛋白9（AQP9）在子痫前期患者胎盘、胎膜组织中的定位、mRNA及表达变化,分析AQP9在子痫前期发病中发挥的作用。方法：选取本院2014年3月至2015年5月收治的100例孕妇作为研究对象,其中有50例为正常晚期妊娠孕妇,将这50例作为对照组;另外50例为子痫前期孕妇（孕周38~42周）,轻度子痫前期孕妇与重度子痫前期孕妇各25例,将这50例子痫前期患者作为观察组。利用免疫组织化学增强聚合物法对AQP9的定位情况进行测定;利用逆转录-聚合酶链反应对AQP9 mRNA进行检测;利用蛋白印迹技术对蛋白的表达水平进行检测;通过使用凝胶蛋白定量分析软件对蛋白灰度值进行测定。结果：（1）AQP9在羊膜上皮细胞、绒毛膜滋养细胞和合体滋养细胞上定位;（2）两组患者的胎膜组织和胎盘中都有AQP9 mRNA表达;（3）观察组胎膜组织、胎膜中AQP9的灰度值都要高于对照组,差异显著,具有统计学意义（P〈0.05）,而且观察组中重度子痫前期患者胎膜组织、胎膜中AQP9的灰度值要高于轻度子痫前期患者,对比差异,具有统计学意义（P〈0.05）。结论：子痫前期患者AQP9的表达水平得到较大程度的提升,AQP9是造成子痫前期发病的主要因素。

Objectives： To observe changes in the location,mRNA and expression of aquaporin- 9（ AQP9） in placenta and fetal membrane of preeclampsia patients,to analyze its role in the pathogenesis of preeclampsia. Methods： 100 pregnant women treated in our hospital from March 2014 to May 2015 were selected including 50 normal third trimester pregnant women as control group and 50 preeclampsia patients（ gestational age of38 to 42 weeks） as observation group,i. e. 25 mild preeclampsia pregnant women and 25 severe ones. Immunohistochemical reinforced polymer was used to determine the location of AQP9,reverse transcription- polymerase chain reaction to test the 9 mRNA,protein imprinting technology to test the protein expression levels,and the gel protein quantitative analysis software for determination of protein grey value. Results： Firstly,the AQP9 was located in amniotic epithelial cells,human chorionic trophocyte and sertoli cells. Secondly,expression of AQP9 was found in the membranes and placenta of both groups. Thirdly,the AQP9 grey value on placenta and fetal membrane in the observation group was higher than the control group,with statistically significant difference（ P〈0. 05）,and the AQP9 grey value on placenta and fetal membrane in patients with severe preeclampsia was also higher than those with mild preeclampsia,with statistically significant difference（ P〈0. 05）. Conclusions： Expression level of AQP9 in patients with preeclampsia increases,indicating AQP9 is the main factor of preeclampsia.