三种波长激光对成纤维细胞Ⅰ型和Ⅲ型前胶原mRNA表达的影响

Effect of three wavelength lasers on mRNA expression of types Ⅰ and Ⅲ procollagen in fibroblasts

目的探讨595nm，755nm和1064nm3种波长激光照射对体外培养的成纤维细胞Ⅰ型和Ⅲ型前胶原mRNA表达水平的影响。方法将体外培养昆明小鼠成纤维细胞分成595nm组、755nm组、1064nm组和无激光照射组（对照组）。应用反转录-聚合酶链反应（RT—PCR）方法测定成纤维细胞Ⅰ型和Ⅲ型前胶原mRNA表达水平。结果1064nm组Ⅰ型前胶原mRNA表达水平比755nm组、595nm组与对照组高（P〈0．05）；755nm组和595nm组的表达水平高于对照组（P〈0．05）；但是755nm组与595nm组间差异无统计学意义（P〉0．05）。1064nm组Ⅲ型前胶原mRNA表达水平比755nm组、595nm组与对照组高（P〈0．05）；755nm组比595nm组和对照组表达高（P〈0．05）；但是595nm组与对照组的差异无统计学意义（P〉0．05）。结论3种波长激光可直接促进成纤维细胞中Ⅰ型和Ⅲ型前胶原mRNA的表达。595nm激光主要促进Ⅰ型前胶原mRNA增加，755nm激光比595nm激光促进更多的Ⅲ型前胶原mRNA表达，1064nm激光促进Ⅰ型和Ⅲ型前胶原mRNA表达最多。

Objective To investigate the effects of different wavelength （595 nm, 755 nm and 1064 nm） lasers on the mRNA expression of types Ⅰ and Ⅲ procollagen in cultured fibroblasts. Methods Fibroblasts from Kunming mice were cultured in vitro. They were divided into 595 nm laser group, 755 nm laser group, 1064 nm laser group and no laser irra diating group. The mRNA expression of the types Ⅰ and Ⅲ procollagen was detected by RT-PCR. Results The mRNA expression level of type Ⅰ procollagen in 1064 nm group was higher than that of 755 nm group, 595 nm group and control group （P〈0.05）. The expression levels of 755 nm group and 595 nm group were higher than that of control group （P〈0.05）. But the difference between 755 nm group and 595 nm group was not statistically significant （P 〉0.05）. The mRNA expression level of type Ⅲ proeollagen in 1064 nm group was higher than that in 755 nm group, 595 nm group and control group （P〈0.05） . 755 nm group had higher expression than 595 nm group and control group （P〈0.05）. But the difference between 595 nm group and the control group was not statistically significant （P〈0.05）. Conclusions Three wavelength lasers can directly promote mRNA expression of types Ⅰ and Ⅲ procollagen in fibroblasts. 595 nm laser mainly promotes mRNA expression of type Ⅰ proeollagen, and 755 nm laser promotes more mRNA expression of type m procollagen than 595 nm laser. The most mRNA expression of types Ⅰ and Ⅲ procollagen is promoted by 1064 nm laser.