莲房原花青素对极低频电磁场致免疫功能损伤的预防作用

Preventive Effects of Lotus Seed Pod Procyanidins on Extremely Low Frequency Electromagnetic Exposure-induced Immune Function Injury

为了探讨莲房原花青素(LSPCs)对极低频电磁场(ELF-EMF)致小鼠免疫功能损伤的影响,通过半体外法研究LSPCs对脾脏淋巴细胞增殖、NK细胞活性以及血浆和肝脏中细胞因子IL-4和INF-γ的影响,初步确定其免疫调节活性。MTT法检测T、B淋巴细胞增殖、乳酸脱氢酶法检测NK细胞活性、ELASA试剂盒检测IL-4和INF-γ浓度。结果表明:与正常组相比,ELF-EMF辐照后胸腺和脾脏指数显著降低(p<0.05,p<0.01),脾脏T、B淋巴细胞增殖率和NK细胞活性显著降低(p<0.01)、IL-4和INF-γ浓度明显降低;而经LSPCs预处理后,与ELF-EMF组相比上述指标均有所改善,其中90 mg/kg LSPCs组,显著提高了胸腺和脾脏指数(p<0.05,p<0.01),能显著地刺激了T、B淋巴细胞的增殖、提高了NK细胞活性(p<0.01),并且促进了血浆和肝脏分泌IL-4和INF-γ。由此显示LSPCs可以有效地预防ELF-EMF致小鼠免疫功能损伤,调节免疫活性。

To explore the effects of lotus seed pod procyanidins （LSPCs） on extremely low frequency electromagnetic field （ELF-EMF） exposure-induced immune function injury in male mice, a senti-in vitro method was used to study the effect of LSPCs on splenic lymphocyte proliferation, natural killer （NK） cell activity, interleukin 4 （IL-4）, and interferon gamma （IFN-γ） in the plasma and liver. In addition, the immunomodulatory activity of LSPCs was preliminarily determined. The proliferation of T and B lymphocytes was measured by a [3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetmzolium bromide] tetrazolium （MTT） reduction assay. NK cell activity was determined by a lactate dehydrogenase （LDH） release assay, and the concentrations of IL-4 and IFN-γ in plasma and liver were measured by an enzyme-linked immunosorbent assay （ELISA） kit. Our experiments indicated that after ELF-EMF radiation, the spleen and thymus indices were significantly decreased （p〈 0.05,p 〈 0.01, respectively） compared to those of the control. MTT and LDH release assays showed that the proliferation ofT and B lymphocytes and NK-cell activity decreased significantly in the ELF-EMF group （p 〈 0.01）. The concentration of IL-4 and IFN-γ decreased significantly in the plasma and liver. However, pretreatment with LSPCs improved the above indicators compared to those of the ELF-EMF group. Especially for the ELF-EMF＋LSPCs90 group, the spleen and thymus indices increased significantly （p 〈 0.05, p 〈 0.01, respectively）, the proliferation of T and B lymphocytes was significantly stimulated, and NK cell activity and secretion of IL-4 and IFN-γ, in the plasma and liver were promoted. In conclusion, LSPCs can effectively prevent the ELF-EMF-induced immune injury and regulate immune activity.