摘要
为了探讨莲房原花青素(LSPCs)对极低频电磁场(ELF-EMF)致小鼠免疫功能损伤的影响,通过半体外法研究LSPCs对脾脏淋巴细胞增殖、NK细胞活性以及血浆和肝脏中细胞因子IL-4和INF-γ的影响,初步确定其免疫调节活性。MTT法检测T、B淋巴细胞增殖、乳酸脱氢酶法检测NK细胞活性、ELASA试剂盒检测IL-4和INF-γ浓度。结果表明:与正常组相比,ELF-EMF辐照后胸腺和脾脏指数显著降低(p<0.05,p<0.01),脾脏T、B淋巴细胞增殖率和NK细胞活性显著降低(p<0.01)、IL-4和INF-γ浓度明显降低;而经LSPCs预处理后,与ELF-EMF组相比上述指标均有所改善,其中90 mg/kg LSPCs组,显著提高了胸腺和脾脏指数(p<0.05,p<0.01),能显著地刺激了T、B淋巴细胞的增殖、提高了NK细胞活性(p<0.01),并且促进了血浆和肝脏分泌IL-4和INF-γ。由此显示LSPCs可以有效地预防ELF-EMF致小鼠免疫功能损伤,调节免疫活性。
To explore the effects of lotus seed pod procyanidins (LSPCs) on extremely low frequency electromagnetic field (ELF-EMF) exposure-induced immune function injury in male mice, a senti-in vitro method was used to study the effect of LSPCs on splenic lymphocyte proliferation, natural killer (NK) cell activity, interleukin 4 (IL-4), and interferon gamma (IFN-γ) in the plasma and liver. In addition, the immunomodulatory activity of LSPCs was preliminarily determined. The proliferation of T and B lymphocytes was measured by a [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetmzolium bromide] tetrazolium (MTT) reduction assay. NK cell activity was determined by a lactate dehydrogenase (LDH) release assay, and the concentrations of IL-4 and IFN-γ in plasma and liver were measured by an enzyme-linked immunosorbent assay (ELISA) kit. Our experiments indicated that after ELF-EMF radiation, the spleen and thymus indices were significantly decreased (p〈 0.05,p 〈 0.01, respectively) compared to those of the control. MTT and LDH release assays showed that the proliferation ofT and B lymphocytes and NK-cell activity decreased significantly in the ELF-EMF group (p 〈 0.01). The concentration of IL-4 and IFN-γ decreased significantly in the plasma and liver. However, pretreatment with LSPCs improved the above indicators compared to those of the ELF-EMF group. Especially for the ELF-EMF+LSPCs90 group, the spleen and thymus indices increased significantly (p 〈 0.05, p 〈 0.01, respectively), the proliferation of T and B lymphocytes was significantly stimulated, and NK cell activity and secretion of IL-4 and IFN-γ, in the plasma and liver were promoted. In conclusion, LSPCs can effectively prevent the ELF-EMF-induced immune injury and regulate immune activity.
出处
《现代食品科技》
EI
CAS
北大核心
2016年第7期1-5,59,共6页
Modern Food Science and Technology
基金
国家自然科学基金(31201456
31371734)
江苏省自然基金(BK2012287)
江苏省"六大人才高峰"(SWYY-022)
江苏省"青蓝工程"
江苏高校优势学科建设工程项目
关键词
LSPCs
ELF-EMF
T
B淋巴细胞
NK细胞
IL-4
INF-Γ
lotus seed pod procyanidins
extremely low frequency electromagnetic field
T, B lymphocytes
natural killer cell
interleukin4
interferon gamma