T-2毒素对不同生长期对虾的急性毒性及其分析方法的比较研究

Acute Toxicity of T-2 Toxin in Shrimp at Different Growth Stages and Comparison of Corresponding Analytical Methods

比较T-2毒素对不同生长期凡纳滨对虾(Litopenaeus vannamei)的急性毒性及其分析方法,明确对虾作为T-2毒素毒性评价的生物标记物的最佳时期,建立T-2毒素对对虾的急性毒性互补评价方法。通过对不同生长期凡纳滨对虾的急性毒性试验,采用寇氏法和概率单位法,计算得到T-2毒素对不同生长期凡纳滨对虾的LD_(50),并通过u检验对两种方法求得的LD_(50)值进行分析比较。结果得到T-2毒素对虾卵、幼虾和成虾的LD_(50)值分别为2.33、1.79和3.34 mg/kg·bw,且经检验两种方法求得的LD_(50)并无显著性差异。研究表明,T-2毒素对成虾的毒害作用最小急性毒性最弱,对幼虾的毒害作用最大急性毒性最强,即该生长期的凡纳滨对虾更适宜做评价T-2毒素急性毒性的生物标志物,同时采用寇氏法和概率单位法相结合的方法得到的LD_(50)值更具准确。

The acute toxicity of T-2 toxin on Litopenaeus vannamei at different growth periods and corresponding analytical methods were compared.Additionally,the optimal period to use shrimps as a biomarker for evaluation of the toxicity of T-2 toxin was determined,and a complementary method for evaluation of the acute toxicity of T-2 toxin on shrimps was established.Acute toxicity tests were performed on L.vannamei at different growth stages.The Spearman-Karber and probability unit methods were used to calculate the lethal dose 50%（LD50）values for T-2 toxin in L.vannamei at different growth stages.The obtained LD50 values were compared by u-tests.The LD50 values for T-2 toxin in shrimp eggs,juvenile shrimps,and adult shrimps were 2.33,1.79,and 3.34 mg/kg of body weight,respectively,and no significant differences were observed between the LD50 values obtained using the two methods.These findings suggested that T-2 toxin exhibited the lowest toxic effect on adult shrimps and the highest toxic effect on juvenile shrimps.Thus,juvenile L.vannamei were more suitable for biomarker analysis when evaluating the acute toxicity of T-2 toxin,and a relatively accurate LD50 value could be obtained using a method combining the Spearman-Karber method and the probability unit method.