月桂叶中黄酮类成分的分析鉴定

Identification of Flavonoids in the Leaves of Laurus nobilis

利用液相色谱-四级杆-飞行时间串联质谱(LC-Q-TOF-MS),结合标准品比较、保留时间对比、多级质谱碎片和相关文献,鉴定了月桂叶黄酮的化学成分。利用液相-三重四级杆串联质谱(LC-MS/MS)在多反应监控模式(MRM)下,分别测定了黄酮化合物的含量。从我国月桂叶中分离鉴定出了16种黄酮化合物,包括牡荆素鼠李糖苷(1658.53μg/g±56.9)、芦丁(19883.54±494.33μg/g)、牡荆素(4430.87±89.72μg/g)、A-型原花青素三聚体(2281.88±78.90μg/g)、表儿茶素(148.10μg/g±2.32)、山奈酚-3-O-芸香糖苷(3491.02±47.98μg/g)、异鼠李素-3-O-芸香糖苷(6643.78±99.04μg/g)、金丝桃苷(11979.66±317.79μg/g)、山奈酚-7-O-葡萄糖苷(738.55μg/g±10.86)、紫云英苷(508.60μg/g±6.96)、槲皮素-3-O-木糖苷(2044.22μg/g±57.65)、异鼠李素-3-O-葡萄糖苷(2079.94μg/g±76.38)、槲皮苷(2079.94μg/g±61.84)、山奈酚-3-O-鼠李糖苷(6412.37±78.65μg/g)、槲皮素(1629.45μg/g±47.65)和山奈酚(364.29μg/g±2.84)。测定了月桂叶黄酮提取物清除DPPH自由基的能力,结果证明,月桂叶黄酮具有良好的清除自由基能力。

Flavonoids in the leaves of Laurus nobilis were identified using liquid chromatography-quadrupole-time of flight mass spectrometry combined with comparison of the retention times and tandem mass spectrometric fragment patterns of standards or from related literature.Flavonoid contents were measured by liquid chromatography-triple quadrupole tandem mass spectrometry in multiple reaction monitoring mode.Sixteen flavonoids were detected in L.nobilis leaves,including vitexin-2-O-rhamnoside（1658.53 ± 56.9 μg/g）,rutin（19,883.54 ± 494.33 μg/g）,vitexin（4430.87 ± 89.72 μg/g）,type-A procyanidin trimer（2281.88 ± 78.90 μg/g）,epicatechin（148.10 ± 2.32 μg/g）,kaempferol-3-O-rutinoside（3491.02 ± 47.98 μg/g）,isorhamnetin-3-O-rutinoside（6643.78 ± 99.04 μg/g）,hyperoside（11,979.66 ± 317.79 μg/g）,kaempferol-7-glucoside（738.55 ± 10.86 μg/g）,astragalin（508.60 ± 6.96 μg/g）,quercetin-3-O-xylopyranoside（2044.22 ± 57.65 μg/g）,isorhamnetin-3-O-glycosidase（2079.94 ± 76.38 μg/g）,quercitrin（2079.94 ± 61.84 μg/g）,kaempferol-3-O-rhamnoside（6412.37 ± 78.65 μg/g）,quercetin（1629.45 ± 47.65 μg/g）,and kaempferol（364.29 ± 2.84 μg/g）.The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities of the extract were determined,and the results clearly demonstrated that flavonoids from L.nobilis possessed significant radical scavenging activities.