IL-2、IL-6与原发性闭角型青光眼视网膜神经纤维层损伤的相关性研究

Correlations between IL-2/IL-6 levels and retinal nerve fiber layer damage in primary angle-closure glaucoma

目的探讨原发性闭角型青光眼外周血IL-2、IL-6水平与视网膜神经纤维层损伤的相关性。方法采用病例对照研究。病例组为临床诊断为原发性闭角型青光眼的患者80例,按眼压正常（10~21 mm Hg）、轻度升高（22~30 mm Hg）、重度升高（31~60 mm Hg）分为A、B、C 3组,同期住院老年性白内障患者40例作为对照组。采用双抗体夹心酶联免疫吸附法测定外周血IL-2、IL-6质量浓度,采用光学相干断层成像和视野计评价视网膜神经纤维层损伤。所得结果采用独立样本t检验,病例组数据进行Dunnett-t检验和相关性分析。结果病例组RNFL（retial nerve fiber layer）厚度、IL-2、IL-6水平均较对照组低,差异有统计学意义（P〈0.05）。A、B、C 3组RNFL厚度、MD、IL-2、IL-6水平差异有统计学意义（P〈0.05）。C组RNFL厚度、IL-2、IL-6水平均低于A组（P〈0.05）,A、B组比较差异无统计学意义（P〉0.05）。病例组眼压与IL-2、IL-6水平、MD及RNFL厚度呈负相关（r=-0.280、-0.237、-0.590、-0.549,P〈0.05）。病例组RNFL厚度与IL-2、IL-6水平、MD呈正相关（r=0.309、0.261、0.590,P〈0.05）。结论外周血IL-2、IL-6水平降低与原发性闭角型青光眼视网膜神经纤维层损伤相关,提示免疫机制可能参与了原发性闭角型青光眼的发病和进展,IL-2、IL-6可能是原发性闭角型青光眼的保护性因素。

This study performed to evaluate the levels of IL-2 and IL-6 in primary acute angle-closureglaucoma（PACG）, and their correlation to retinal nerve fiber layer（RNFL） damage. Tatol of 80 cases of PACG inclinical diagnosis were included, and divided into three groups according to intraocular pressur（IOP）： group A（IOP： 10-21 mm Hg）, group B（IOP： 22-30 mm Hg） and group C（IOP： 31-60 mm Hg）. 40 cases with age-relatedcataract hosiplized in the same period were selected as control group. The levels of IL-2 and IL-6 in the peripheralblood of subjects were determined with double-antibody sandwich enzyme-linked immunosorbent assay（ELISA）,while the RNFL damage was evaluated by optical coherence tomography and automatic perimetry. Data showed thatthe RNFL thickness, IL-2 and IL-6 levels in case group were significantly lower than those of control group（P〈0.05）; the levels of IL-2, IL-6, mean defect（MD）, and RNFL thickness were significant different in groups A, B andC（P〈0.05）. The levels of IL-2, IL-6 and RNFL thickness in group C were significantly lower than those in groupA（P〈0.05）, but there was no significant difference between groups A and B（P〉0.05）. The levels of IL-2, IL-6, MDand RNFL thickness in experimental group were negatively correlated with IOP（correlationcoefficient=-0.280,-0.237,-0.590,-0.549, P〈0.05）. The levesls of IL-2, IL-6 and MD in experimental groupwere positively correlated with RNFL thickness（correlation coefficient=0.309, 0.261, 0.590, P〈0.05）. Inconclusion, the decresase of IL-2 and IL-6 associate with the RNFL damage in PACG, indicating that the immunemechanisms may be involved in the pathogenesis and progression of PACG. Thus IL-2 and IL-6 may be theprotective factors against PACG.