80株食源性金黄色葡萄球菌药敏结果分析

Analysis of susceptibility test results of 80 strains of food borne Staphylococcus aureus

目的研究北京市昌平区2011年-2014年分离获得的80株食源性金黄色葡萄球菌药物敏感性和耐药性,为该地区金黄色葡萄球菌引起的食源性疾病预防及治疗提供参考。方法依据GB 4789.10—2010进行菌株分离和鉴定,用VITEK 2 Compact进行药敏试验,应用检测试剂盒进行β-内酰胺酶检测。结果金黄色葡萄球菌对莫西沙星、喹努普汀、利奈唑胺、万古霉素、替加环素和呋喃妥因的敏感率为100.0%,对青霉素耐药率最高(86.2%),其中多重耐药率为53.7%,检出2株耐苯唑西林(MRSA)金黄色葡萄球菌。68株β-内酰胺酶阳性株除青霉素100.0%耐药外,红霉素耐药率最高,为47.1%,其中多重耐药率为57.4%,12株β-内酰胺酶阴性株,多重耐药率为33.3%,所有菌株对青霉素敏感。结论昌平区食源性金黄色葡萄球菌存在耐药和多重耐药现象,应根据药物敏感性试验结果有针对性选择抗菌药物,减少耐药菌株的产生。

Objective To study the drug susceptibility and resistance of 80 Staphylococcus aureus strains from Changping district of Beijing during 2011- 2014,so as to provide reference for the control and prevention of food borne disease caused by Staphylococcus aureus. Methods Staphylococcus aureus strains were isolated for identification according to the standard GB4789. 10—2010,using VITEK 2 Compact for drug susceptibility test,and using rapid detection kit for the detection of β- lactamase. Results All of food borne Staphylococcus aureus strains showed 100. 0% susceptibility to moxifloxacin,leptin / dafoe leptin,linezolid,vancomycin,tigecycline and nitrofurantoin. Resistant rate to penicillin was the highest（ 86. 2%）,in which multi- drug resistance accounted for 53. 7%. Two strains were methicillin- resistant Staphylococcus aureus（ MRSA）. 68 strains of β- lactamase positive were 100. 0% resistant to penicillin except penicillin,and the highest resistant rate was 47. 1% to erythromycin. The multiple resistant rate was 57. 4%. 12 β- lactamase negative strains were multiple drug- resistant,with multiple resistant rate of 33. 3%. All strains were sensitive to penicillin. Conclusion The resistance and multiple antibiotic resistance of Staphylococcus aureus were serious in Changping district of Beijing,so we should select antibiotics according to the results of drug sensitivity test,so as to reduce the occurrance of resistant strains.